Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE)

DNA carries biological information in a form that must be precisely copied and transmitted to all progeny cells. Occasionally, changes in the sequence of a gene may occur during the replication process. These ''genetic mistakes"' are known as mutations. Mutations can be identifie...

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Main Author:	Phang, Beng Hooi.
Other Authors:	Tan, Ene Choo
Format:	Theses and Dissertations
Language:	English
Published:	2009
Subjects:	DRNTU::Science::Biological sciences::Microbiology::Virology
Online Access:	http://hdl.handle.net/10356/20341
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Institution:	Nanyang Technological University
Language:	English

id	sg-ntu-dr.10356-20341
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spelling	sg-ntu-dr.10356-203412020-11-01T06:25:48Z Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE) Phang, Beng Hooi. Tan, Ene Choo National Institute of Education DRNTU::Science::Biological sciences::Microbiology::Virology DNA carries biological information in a form that must be precisely copied and transmitted to all progeny cells. Occasionally, changes in the sequence of a gene may occur during the replication process. These ''genetic mistakes"' are known as mutations. Mutations can be identified by physical, chemical or enzymatic means. In this study, mutations in the human (3-globin gene and HBV pre-core gene were used to evaluate the sensitivity of Denaturing Gradiënt Gel Electrophoresis (DGGE). DNA sequencing was then performed to verify and confirm the unknown mutations in the human p-g!obin gene as well as the HBV pre-core gene. Master of Science 2009-12-14T09:35:19Z 2009-12-14T09:35:19Z 1997 1997 Thesis http://hdl.handle.net/10356/20341 en NANYANG TECHNOLOGICAL UNIVERSITY 165 p. application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	DRNTU::Science::Biological sciences::Microbiology::Virology
spellingShingle	DRNTU::Science::Biological sciences::Microbiology::Virology Phang, Beng Hooi. Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE)
description	DNA carries biological information in a form that must be precisely copied and transmitted to all progeny cells. Occasionally, changes in the sequence of a gene may occur during the replication process. These ''genetic mistakes"' are known as mutations. Mutations can be identified by physical, chemical or enzymatic means. In this study, mutations in the human (3-globin gene and HBV pre-core gene were used to evaluate the sensitivity of Denaturing Gradiënt Gel Electrophoresis (DGGE). DNA sequencing was then performed to verify and confirm the unknown mutations in the human p-g!obin gene as well as the HBV pre-core gene.
author2	Tan, Ene Choo
author_facet	Tan, Ene Choo Phang, Beng Hooi.
format	Theses and Dissertations
author	Phang, Beng Hooi.
author_sort	Phang, Beng Hooi.
title	Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE)
title_short	Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE)
title_full	Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE)
title_fullStr	Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE)
title_full_unstemmed	Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE)
title_sort	detection of mutations and polymorphisms in the human b-globin gene and hepatitis b virus precore region by denaturing gradient gel electrophoresis (dgge)
publishDate	2009
url	http://hdl.handle.net/10356/20341
_version_	1683494403533963264

Detection of mutations and polymorphisms in the human B-globin gene and hepatitis B virus precore region by denaturing gradient gel electrophoresis (DGGE)

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