Gene-primer a PCR primer design program

Gene-primer a PCR primer design program

Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reactions. In this project, we develop GENE-PRIMER, a genome- specific PCR primer design program that is amenable to a genome-wide scale. To achieve this, we incorporated various parameters with biological...

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Main Author: Kok, Wui Lim.
Other Authors: Kwoh, Chee Keong
Format: Theses and Dissertations
Published: 2008
Subjects:
DRNTU::Engineering::Computer science and engineering::Computer applications::Life and medical sciences
Online Access:http://hdl.handle.net/10356/2674
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-26742023-03-04T00:31:59Z Gene-primer a PCR primer design program Kok, Wui Lim. Kwoh, Chee Keong School of Computer Engineering DRNTU::Engineering::Computer science and engineering::Computer applications::Life and medical sciences Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reactions. In this project, we develop GENE-PRIMER, a genome- specific PCR primer design program that is amenable to a genome-wide scale. To achieve this, we incorporated various parameters with biological significance into our program, namely, primer length, melting temperature of primers ,guanine/cytosine (GC) content of primer, homopolymeric runs in primer and self- hybridization tendency of primer. In addition, BLAST algorithm is utilized for the purpose of primer specificity check. In summary, selected primers adhered to both physico-chemical criteria and also display specificity to intended binding site in the genome. Master of Science (Bioinformatics) 2008-09-17T09:07:32Z 2008-09-17T09:07:32Z 2005 2005 Thesis http://hdl.handle.net/10356/2674 Nanyang Technological University application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
topic DRNTU::Engineering::Computer science and engineering::Computer applications::Life and medical sciences
spellingShingle DRNTU::Engineering::Computer science and engineering::Computer applications::Life and medical sciences
Kok, Wui Lim.
Gene-primer a PCR primer design program
description Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reactions. In this project, we develop GENE-PRIMER, a genome- specific PCR primer design program that is amenable to a genome-wide scale. To achieve this, we incorporated various parameters with biological significance into our program, namely, primer length, melting temperature of primers ,guanine/cytosine (GC) content of primer, homopolymeric runs in primer and self- hybridization tendency of primer. In addition, BLAST algorithm is utilized for the purpose of primer specificity check. In summary, selected primers adhered to both physico-chemical criteria and also display specificity to intended binding site in the genome.
author2 Kwoh, Chee Keong
author_facet Kwoh, Chee Keong
Kok, Wui Lim.
format Theses and Dissertations
author Kok, Wui Lim.
author_sort Kok, Wui Lim.
title Gene-primer a PCR primer design program
title_short Gene-primer a PCR primer design program
title_full Gene-primer a PCR primer design program
title_fullStr Gene-primer a PCR primer design program
title_full_unstemmed Gene-primer a PCR primer design program
title_sort gene-primer a pcr primer design program
publishDate 2008
url http://hdl.handle.net/10356/2674
_version_ 1759858371013378048

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