Ceramics-based electrochemical uric acid biosensor

This project explores the development of a novel disposable biosensor based on coating Fe2O3 and Fe3O4 on commercially available screen-printed electrodes. It looks into the preparation, characterisation and measurement of current values from the electrochemical reaction at the biosensor which is us...

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Main Author: Phua, Dindson Jun Liang.
Other Authors: Raju Vijayaraghavan Ramanujan
Format: Final Year Project
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/10356/35516
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-355162023-03-04T15:42:09Z Ceramics-based electrochemical uric acid biosensor Phua, Dindson Jun Liang. Raju Vijayaraghavan Ramanujan School of Materials Science and Engineering DRNTU::Engineering::Materials::Biomaterials This project explores the development of a novel disposable biosensor based on coating Fe2O3 and Fe3O4 on commercially available screen-printed electrodes. It looks into the preparation, characterisation and measurement of current values from the electrochemical reaction at the biosensor which is useful for the detection of uric acid. The combination of carbon as the substrate, the overlying Fe3O4 layer and the conductive gold coating showed good adsorption properties for the uricase enzyme molecules and yielded good results. 5μl of 5 mg/ml uricase was immobilised onto the electrode surfaces by physical adsorption or physiosorption for a period of 24 hours at 4oC. The electrode was tested against a uric acid concentration of 0.006 mol/L in pH 7.4 Phosphate Buffer Saline (0.1M). It was observed that the current increased by a value of about 0.03 μA, and this presents us with a fair electrochemical response for the detection of uric acid. This approach however can be further refined and improved upon for use in clinical applications and even for detection of other immunological agents. Bachelor of Engineering (Materials Engineering) 2010-04-20T03:12:17Z 2010-04-20T03:12:17Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/35516 en Nanyang Technological University 35 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Phua, Dindson Jun Liang.
Ceramics-based electrochemical uric acid biosensor
description This project explores the development of a novel disposable biosensor based on coating Fe2O3 and Fe3O4 on commercially available screen-printed electrodes. It looks into the preparation, characterisation and measurement of current values from the electrochemical reaction at the biosensor which is useful for the detection of uric acid. The combination of carbon as the substrate, the overlying Fe3O4 layer and the conductive gold coating showed good adsorption properties for the uricase enzyme molecules and yielded good results. 5μl of 5 mg/ml uricase was immobilised onto the electrode surfaces by physical adsorption or physiosorption for a period of 24 hours at 4oC. The electrode was tested against a uric acid concentration of 0.006 mol/L in pH 7.4 Phosphate Buffer Saline (0.1M). It was observed that the current increased by a value of about 0.03 μA, and this presents us with a fair electrochemical response for the detection of uric acid. This approach however can be further refined and improved upon for use in clinical applications and even for detection of other immunological agents.
author2 Raju Vijayaraghavan Ramanujan
author_facet Raju Vijayaraghavan Ramanujan
Phua, Dindson Jun Liang.
format Final Year Project
author Phua, Dindson Jun Liang.
author_sort Phua, Dindson Jun Liang.
title Ceramics-based electrochemical uric acid biosensor
title_short Ceramics-based electrochemical uric acid biosensor
title_full Ceramics-based electrochemical uric acid biosensor
title_fullStr Ceramics-based electrochemical uric acid biosensor
title_full_unstemmed Ceramics-based electrochemical uric acid biosensor
title_sort ceramics-based electrochemical uric acid biosensor
publishDate 2010
url http://hdl.handle.net/10356/35516
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