Regulation of coronavirus gene expression by the 5'-untranslated region.
The regulatory properties of the 5’-untranslated region (5’-UTR) of the avian infectious bronchitis virus (IBV) were investigated through designing of plasmids which contained different mutations within the 5’-UTR: full-length (pUTR-wt), 362- nucleotides (nts) deleted (pUTR-362del) and stem-loop...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/38610 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The regulatory properties of the 5’-untranslated region (5’-UTR) of the avian
infectious bronchitis virus (IBV) were investigated through designing of plasmids
which contained different mutations within the 5’-UTR: full-length (pUTR-wt), 362-
nucleotides (nts) deleted (pUTR-362del) and stem-loop 1 (SL1) disrupted (pSL1-
dsmut). By inserting a Firefly luciferase gene into the constructs, differences in gene
expression were determined by expressing these plasmids in a mammalian cell
system through transfection and measuring the expression levels through luciferase
assays and immunoblotting. As a result of deleting 362 nts from the 5’-UTR, a 2-fold
increment in gene expression was observed, which confirmed the finding made by
Liu et al. (1998). Furthermore, it was found that a disruption of SL1 could result in a
slight decrease in gene expression. Hence, these suggest that nts 363 to 528 in the
5’-UTR might be important in enhancing gene expression while SL1 could contribute
itself as an initiator for gene expression in IBV. |
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