Protein adsorption on mesoporous particles

• Main Author: Tan, Zhi Hong.
• Other Authors: Susanna Leong Su Jan
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnology
• Online Access: http://hdl.handle.net/10356/39432
• Institution: Nanyang Technological University

The objective of this project is to qualitatively and quantitatively study protein adsorption behaviour on ion exchange chromatography beads. Knowing the protein-particle interaction facilitates the study and choice of resin chemistry required for better chromatographic performance and resolution towards different proteins. With better understanding of the activity at the molecular level, effects of conformational differences on adsorption behaviour may then be explained. The effects of resin properties such as porosity and size, as well as resin chemistry on protein adsorption were investigated. Additionally, the effect of the proteins’ conformational states (i.e. denatured versus native) on their adsorption behaviour was also studied. Specifically, the static adsorption capacities and adsorption profile of different resins were compared using BSA and AFP as model proteins. Protein concentration was quantified using the Bradford assay.