Calcium regulation of glucagon-like peptide-1 secretion.

Glucagon-like peptide (GLP-1), a hormone secreted from enteroendocrine L-cells in response to nutrient ingestion. Because of its strong potentiating effect on insulin release, GLP-1 now becomes a basis for new class diabetes treatment strategies. However, molecular mechanism of GLP-1 secretion re...

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Main Author: Chua, Sharon Chia Ling.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2010
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Online Access:http://hdl.handle.net/10356/39459
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-394592023-02-28T18:06:15Z Calcium regulation of glucagon-like peptide-1 secretion. Chua, Sharon Chia Ling. School of Biological Sciences Dr Han Weiping DRNTU::Science::Biological sciences::Molecular biology Glucagon-like peptide (GLP-1), a hormone secreted from enteroendocrine L-cells in response to nutrient ingestion. Because of its strong potentiating effect on insulin release, GLP-1 now becomes a basis for new class diabetes treatment strategies. However, molecular mechanism of GLP-1 secretion regulation has remained elusive. It is known that GLP-1 secretion is calcium (Ca2+) -dependent but extent of Ca2+ regulation is not established. The aim of this study was to examine the relation between Ca2+ response and GLP-1 secretion and search for the proteins serving as Ca2+ sensors. In β-cells, the identities of Ca2+ sensors only recently started to emerge. Here we tested whether synaptotagmin-2 (Syt2) operates Ca2+ sensing in GLP-1 release by generating Syt2 knockdown (KD) GLUTag cells. We characterized dose-dependence for Ca2+ response to stimulus. For glucose stimulation, GLP-1 secretion reflected the amplitude of Ca2+ increase. In contrast, glutamine had a strong non- Ca2+ dependent component in addition to its Ca2+ -dependent release. Syt2 KD resulted in a reduction of GLP-1 release, however the subcellular localization showed very little co-localization with GLP-1 containing granules. Therefore, Syt2 may directly operate Ca2+ sensing of readily releasable pool of GLP- 1 granules and may also regulate GLP-1 secretion indirectly through regulation of granule trafficking. Bachelor of Science in Biological Sciences 2010-05-24T08:54:24Z 2010-05-24T08:54:24Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39459 en Nanyang Technological University 33 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Molecular biology
spellingShingle DRNTU::Science::Biological sciences::Molecular biology
Chua, Sharon Chia Ling.
Calcium regulation of glucagon-like peptide-1 secretion.
description Glucagon-like peptide (GLP-1), a hormone secreted from enteroendocrine L-cells in response to nutrient ingestion. Because of its strong potentiating effect on insulin release, GLP-1 now becomes a basis for new class diabetes treatment strategies. However, molecular mechanism of GLP-1 secretion regulation has remained elusive. It is known that GLP-1 secretion is calcium (Ca2+) -dependent but extent of Ca2+ regulation is not established. The aim of this study was to examine the relation between Ca2+ response and GLP-1 secretion and search for the proteins serving as Ca2+ sensors. In β-cells, the identities of Ca2+ sensors only recently started to emerge. Here we tested whether synaptotagmin-2 (Syt2) operates Ca2+ sensing in GLP-1 release by generating Syt2 knockdown (KD) GLUTag cells. We characterized dose-dependence for Ca2+ response to stimulus. For glucose stimulation, GLP-1 secretion reflected the amplitude of Ca2+ increase. In contrast, glutamine had a strong non- Ca2+ dependent component in addition to its Ca2+ -dependent release. Syt2 KD resulted in a reduction of GLP-1 release, however the subcellular localization showed very little co-localization with GLP-1 containing granules. Therefore, Syt2 may directly operate Ca2+ sensing of readily releasable pool of GLP- 1 granules and may also regulate GLP-1 secretion indirectly through regulation of granule trafficking.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Chua, Sharon Chia Ling.
format Final Year Project
author Chua, Sharon Chia Ling.
author_sort Chua, Sharon Chia Ling.
title Calcium regulation of glucagon-like peptide-1 secretion.
title_short Calcium regulation of glucagon-like peptide-1 secretion.
title_full Calcium regulation of glucagon-like peptide-1 secretion.
title_fullStr Calcium regulation of glucagon-like peptide-1 secretion.
title_full_unstemmed Calcium regulation of glucagon-like peptide-1 secretion.
title_sort calcium regulation of glucagon-like peptide-1 secretion.
publishDate 2010
url http://hdl.handle.net/10356/39459
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