Cell culture optimization for increased soluble expression of hepatitis B virus X protein.

• Main Author: Lim, Pei Yu.
• Other Authors: Susanna Leong Su Jan
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnology
• Online Access: http://hdl.handle.net/10356/39523
• Institution: Nanyang Technological University

Economical production of proteins is made possible by recombinant protein technology. However, recombinant proteins are frequently expressed as inclusion bodies in E. coli cells. This is unfavourable as inclusion bodies usually contain incorrectly folded and biologically inactive protein. Therefore,...