Ligand effect on cell and bacterial adhesion

The pathogenesis of infections is due to adhesion of bacteria onto biomaterial surfaces. Surface modification would provide these materials with multiple coating functions such as anti-adhesive, antimicrobial, antibacterial and biocide. The modified surfaces exhibit reduced bacterial and cell adhesi...

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Main Author: Char, Cassandra Wai Han.
Other Authors: Chan Vincent
Format: Final Year Project
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/10356/39626
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-396262023-03-03T15:33:29Z Ligand effect on cell and bacterial adhesion Char, Cassandra Wai Han. Chan Vincent School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology The pathogenesis of infections is due to adhesion of bacteria onto biomaterial surfaces. Surface modification would provide these materials with multiple coating functions such as anti-adhesive, antimicrobial, antibacterial and biocide. The modified surfaces exhibit reduced bacterial and cell adhesion. The lack of interactions between cells and biomaterial surfaces is also another cause for foreign body reactions. Cell-recognition motives, such as RGD and collagen, could be immbolised to facilitate cell adhesion. In this study, the layer-by-layer technique was used to fabricate the anti-adhesive dextran sulfate/chitosan polyelectrolyte multilayer (PEM) assembly. RGD and collagen were then grafted onto the PEM. The effect of RGD and collagen on cell adhesion was investigated through 3T3 fibroblast cell seeding. In addition, the abilities of these protein-coated surfaces on Escherichia coli (E. coli) Staphylococcus aureus (S. aureus) adhesion were studied. The results showed that the PEM without proteins, exhibited effective anti-adhesive ability, inhibiting both cell and bacterial adhesion. RGD-grafted surfaces were shown to have enhanced the adherence of cells, while successfully impeded the adhesion of E. coli and S.aureus. Collagen-grafted surfaces, on the other hand, showed significant adhesion to both cell and S. aureus, while inhibited the adhesion of E. coli. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2010-06-01T09:22:52Z 2010-06-01T09:22:52Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39626 en Nanyang Technological University 49 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biotechnology
spellingShingle DRNTU::Engineering::Chemical engineering::Biotechnology
Char, Cassandra Wai Han.
Ligand effect on cell and bacterial adhesion
description The pathogenesis of infections is due to adhesion of bacteria onto biomaterial surfaces. Surface modification would provide these materials with multiple coating functions such as anti-adhesive, antimicrobial, antibacterial and biocide. The modified surfaces exhibit reduced bacterial and cell adhesion. The lack of interactions between cells and biomaterial surfaces is also another cause for foreign body reactions. Cell-recognition motives, such as RGD and collagen, could be immbolised to facilitate cell adhesion. In this study, the layer-by-layer technique was used to fabricate the anti-adhesive dextran sulfate/chitosan polyelectrolyte multilayer (PEM) assembly. RGD and collagen were then grafted onto the PEM. The effect of RGD and collagen on cell adhesion was investigated through 3T3 fibroblast cell seeding. In addition, the abilities of these protein-coated surfaces on Escherichia coli (E. coli) Staphylococcus aureus (S. aureus) adhesion were studied. The results showed that the PEM without proteins, exhibited effective anti-adhesive ability, inhibiting both cell and bacterial adhesion. RGD-grafted surfaces were shown to have enhanced the adherence of cells, while successfully impeded the adhesion of E. coli and S.aureus. Collagen-grafted surfaces, on the other hand, showed significant adhesion to both cell and S. aureus, while inhibited the adhesion of E. coli.
author2 Chan Vincent
author_facet Chan Vincent
Char, Cassandra Wai Han.
format Final Year Project
author Char, Cassandra Wai Han.
author_sort Char, Cassandra Wai Han.
title Ligand effect on cell and bacterial adhesion
title_short Ligand effect on cell and bacterial adhesion
title_full Ligand effect on cell and bacterial adhesion
title_fullStr Ligand effect on cell and bacterial adhesion
title_full_unstemmed Ligand effect on cell and bacterial adhesion
title_sort ligand effect on cell and bacterial adhesion
publishDate 2010
url http://hdl.handle.net/10356/39626
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