Bio-sorption of proteins

• Main Author: Chua, John Tze Yong.
• Other Authors: Wang Kean
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Engineering
• Online Access: http://hdl.handle.net/10356/39638
• Institution: Nanyang Technological University

The aim of this project is to investigate the feasibility of adsorption of lysozyme from aqueous solution onto chitosan hydrogel beads and its derivatives. In this project, native chitosan hydrogel beads, GLU-chitosan beads and ECH-chitosan beads and are tested for adsorption of lysozyme. Crosslinking ratios of 0.2, 0.5, 0.7 and 1.0 are used for each chitosan hydrogel derivative across pH range of 4, 5, 5.5, 6, 9 and 10.5. All three types of chitosan beads are able to adsorb lysozyme. The adsorption isotherm data for adsorption of lysozyme at various pH were fitted to non-linear Langmuir isotherm model. The study showed low conformity to non linear Langmuir isotherm model of lysozyme onto all three types of chitosan beads at acidic pH range and high conformity to non linear Langmuir isotherm model of lysozyme onto all three types of chitosan beads near the isoelectric point of lysozyme(pH 10.9). SEM images shows high adsorption for all three types of beads at high alkaline region. The maximum adsorption capacity was the highest for all three type of beads at pH 10.5 with 306.25mg/g for native chitosan hydrogel beads, 515mg/g for ECH-Chitosan beads and 249mg/g for GLU-Chitosan beads