Electrospinning of 3D scaffold

Two common problems in the fabrication of tissue engineered scaffolds using electrospinning techniques are poor cellular infiltration into the scaffold and thin dimension of the scaffold which limits its application. In this work, we proposed a novel method to fabricate 3D scaffold to address the...

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Main Author: Tee, Chong Yen.
Other Authors: Tan Lay Poh
Format: Final Year Project
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/10356/39784
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-397842023-03-04T15:32:05Z Electrospinning of 3D scaffold Tee, Chong Yen. Tan Lay Poh School of Materials Science and Engineering DRNTU::Engineering::Materials Two common problems in the fabrication of tissue engineered scaffolds using electrospinning techniques are poor cellular infiltration into the scaffold and thin dimension of the scaffold which limits its application. In this work, we proposed a novel method to fabricate 3D scaffold to address these issues. We also suggest to further enhance the properties of our scaffold by surface modifying it. The novel method which we employed to produce our 3D scaffold was a modified electrospinning technique. Scaffold was collected on a needle rather than a flat collector plate. A spherical scaffold with diameter approximately 1.5cm could be collected in half an hour time. Scaffold was then surface modified to incorporate gelatin onto the scaffold. The contact angle testing showed that surface modified scaffold had an extremely high hydrophilicity with 0° contact angle compared to non modified scaffold with 111.60° contact angle. Furthermore, mercury porosimetry test showed that the scaffold produced by our method had pores with diameters over 50μm and porosity of over 90%. These characteristic allowed our scaffold to achieve cell infiltration depth of more than 5mm or 100% cell infiltration. This level of cell infiltration had never been reported in any of the research paper yet. Bachelor of Engineering (Materials Engineering) 2010-06-04T02:38:48Z 2010-06-04T02:38:48Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39784 en Nanyang Technological University 50 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials
spellingShingle DRNTU::Engineering::Materials
Tee, Chong Yen.
Electrospinning of 3D scaffold
description Two common problems in the fabrication of tissue engineered scaffolds using electrospinning techniques are poor cellular infiltration into the scaffold and thin dimension of the scaffold which limits its application. In this work, we proposed a novel method to fabricate 3D scaffold to address these issues. We also suggest to further enhance the properties of our scaffold by surface modifying it. The novel method which we employed to produce our 3D scaffold was a modified electrospinning technique. Scaffold was collected on a needle rather than a flat collector plate. A spherical scaffold with diameter approximately 1.5cm could be collected in half an hour time. Scaffold was then surface modified to incorporate gelatin onto the scaffold. The contact angle testing showed that surface modified scaffold had an extremely high hydrophilicity with 0° contact angle compared to non modified scaffold with 111.60° contact angle. Furthermore, mercury porosimetry test showed that the scaffold produced by our method had pores with diameters over 50μm and porosity of over 90%. These characteristic allowed our scaffold to achieve cell infiltration depth of more than 5mm or 100% cell infiltration. This level of cell infiltration had never been reported in any of the research paper yet.
author2 Tan Lay Poh
author_facet Tan Lay Poh
Tee, Chong Yen.
format Final Year Project
author Tee, Chong Yen.
author_sort Tee, Chong Yen.
title Electrospinning of 3D scaffold
title_short Electrospinning of 3D scaffold
title_full Electrospinning of 3D scaffold
title_fullStr Electrospinning of 3D scaffold
title_full_unstemmed Electrospinning of 3D scaffold
title_sort electrospinning of 3d scaffold
publishDate 2010
url http://hdl.handle.net/10356/39784
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