Experimental and theoretical study of matrix-assisted refolding

• Main Author: Zhang, Chun Yan
• Other Authors: Arvind Rajendran
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnology
• Online Access: http://hdl.handle.net/10356/39800
• Institution: Nanyang Technological University

Inclusion Bodies (IBs), which are dense, insoluble, biologically inactive protein aggregates, are commonly produced in industrial scale protein expression. Matrix-assisted refolding (MAR), or On-Column Refolding (OCR), is a process which transforms IBs from their aggregated form to the correctly fol...