Typing of poultry Salmonella enteritidis and Salmonella typhimurium isolates by pulsed-field gel electrophoresis.

• Main Author: Lim, Yu Qian.
• Other Authors: School of Biological Sciences
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Science::Biological sciences::Microbiology::Bacteria
• Online Access: http://hdl.handle.net/10356/39815
• Institution: Nanyang Technological University

Salmonella enterica subsp. enterica (S.) has been the most common pathogen causing gastroenteritidis in humans for many years. Infections by Salmonella are more often acquired by consumption of contaminated sources, especially poultry and eggs. With its ability to infect a broad host range, Salmonella will continue to post a threat to public health. The aim of this study is to investigate the clonal relationship and diversity among Salmonella isolates. In an effort to characterize the 62 S. Enteritidis and 20 S. Typhimurium isolates, PFGE and phage typing were employed for better discrimination. XbaI-generated PFGE patterns were analyzed with the Bionumerics software. The predominant PFGE pattern for Salmonella Enteritidis and Salmonella Typhimurium are En3 and Ty1, respectively. The collective PFGE and phage type patterns were then categorized into clusters and the prevalent subtypes were identified based on their year. The results suggested that S. Enteritidis strains are less genetically diverse as compared to S. Typhimurium, which coincides with various prior studies. Finally, PFGE and phage typing could serve as steadfast and strong discrimination tools for epidemiology investigations and surveillance of Salmonella.