Convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property.

Oxidized dextran and chitosan were alternatively deposited in a layer-by-layer way onto aminolyzed glass to construct anti-adhesive multilayer assembly. A method to assemble the layers via covalent interaction in a cheap and non-toxic method was developed in this project. The contact-angle, UV-visib...

Full description

Saved in:
Bibliographic Details
Main Author: Liu, Xuan.
Other Authors: Chan Vincent
Format: Final Year Project
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/10356/39948
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-39948
record_format dspace
spelling sg-ntu-dr.10356-399482023-03-03T15:34:43Z Convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property. Liu, Xuan. Chan Vincent School of Chemical and Biomedical Engineering DRNTU::Engineering::Materials::Biomaterials DRNTU::Engineering::Chemical engineering::Biotechnological production Oxidized dextran and chitosan were alternatively deposited in a layer-by-layer way onto aminolyzed glass to construct anti-adhesive multilayer assembly. A method to assemble the layers via covalent interaction in a cheap and non-toxic method was developed in this project. The contact-angle, UV-visible spectroscopy and atomic force microscopy data verified the progressive buildup of the multilayer assembly. The results of initial adhesion of fibroblast cell, Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) on the assemblies with different layer number and outermost layer were tested. It showed that for both oxidized dextran and chitosan as outermost layer, the anti-adhesive effect against fibroblast cell increased with increasing of layer number. Anti-adhesive property against E.coli depended on the outermost layer and oxidized dextran as outermost layer gave a 99% reduction of adhesive bacteria when reaching 10 bilayer. However, all types of assemblies showed no anti-adhesive effect against S.aureus regardless of layer number and outermost layer. Such an easy and shape-independent method to prepare anti-adhesive surface may have good potential for surface modification of medical devices. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2010-06-08T04:42:12Z 2010-06-08T04:42:12Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39948 en Nanyang Technological University 44 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
DRNTU::Engineering::Chemical engineering::Biotechnological production
spellingShingle DRNTU::Engineering::Materials::Biomaterials
DRNTU::Engineering::Chemical engineering::Biotechnological production
Liu, Xuan.
Convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property.
description Oxidized dextran and chitosan were alternatively deposited in a layer-by-layer way onto aminolyzed glass to construct anti-adhesive multilayer assembly. A method to assemble the layers via covalent interaction in a cheap and non-toxic method was developed in this project. The contact-angle, UV-visible spectroscopy and atomic force microscopy data verified the progressive buildup of the multilayer assembly. The results of initial adhesion of fibroblast cell, Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) on the assemblies with different layer number and outermost layer were tested. It showed that for both oxidized dextran and chitosan as outermost layer, the anti-adhesive effect against fibroblast cell increased with increasing of layer number. Anti-adhesive property against E.coli depended on the outermost layer and oxidized dextran as outermost layer gave a 99% reduction of adhesive bacteria when reaching 10 bilayer. However, all types of assemblies showed no anti-adhesive effect against S.aureus regardless of layer number and outermost layer. Such an easy and shape-independent method to prepare anti-adhesive surface may have good potential for surface modification of medical devices.
author2 Chan Vincent
author_facet Chan Vincent
Liu, Xuan.
format Final Year Project
author Liu, Xuan.
author_sort Liu, Xuan.
title Convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property.
title_short Convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property.
title_full Convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property.
title_fullStr Convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property.
title_full_unstemmed Convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property.
title_sort convalent assembly oxidized dextran/chitosan multilayer and its anti-adhesive property.
publishDate 2010
url http://hdl.handle.net/10356/39948
_version_ 1759854619784118272