Fluorescence enhancement of immunoarrays by gold and silica nanoparticles

High-throughput protein microarrays have advanced scientific study into previously very complicated fields such as identification of diseases related proteins and their respective functions. However, they are still not as successful as DNA microarrays in terms of practical usage in medical field. On...

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Main Author: Ramoo, Jeevaneswaran
Other Authors: Li Changming
Format: Final Year Project
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/10356/40169
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-401692023-03-03T15:32:25Z Fluorescence enhancement of immunoarrays by gold and silica nanoparticles Ramoo, Jeevaneswaran Li Changming School of Chemical and Biomedical Engineering DRNTU::Engineering::Nanotechnology High-throughput protein microarrays have advanced scientific study into previously very complicated fields such as identification of diseases related proteins and their respective functions. However, they are still not as successful as DNA microarrays in terms of practical usage in medical field. One of the many reasons is due to low detection signal from microarrays. Hence, they are incapable of producing high quality data from small samples of proteins which is frequent in clinical environment. Hence, this project aim is to improve the fluorescence signal from immunoarrays, a subset of protein microarrays. Two different mechanisms, Surface Plasmon Resonance and Enlarged Receptor Surface, were explored to improve fluorescence signal of immunoarrays. For these mechanisms, gold nanoparticles and silica nanoparticles were needed and they were synthesized by Turkevich method and Stober method respectively. Both mechanisms successfully enhanced fluorescence signals. Surface Enlarged receptor method with silica nanoparticles immobilized on microarray substrate enhanced fluorescence signal 7.3 times. On the other hand, silica coated gold nanoparticles enhanced fluorescence signal by 2.75 times in solution. By no means, these are the best possible enhancements as parameters affecting both mechanisms can be optimized further to produce higher fluorescence signal which will enable detection of protein samples of low concentration. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2010-06-11T03:23:11Z 2010-06-11T03:23:11Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/40169 en Nanyang Technological University 89 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Nanotechnology
spellingShingle DRNTU::Engineering::Nanotechnology
Ramoo, Jeevaneswaran
Fluorescence enhancement of immunoarrays by gold and silica nanoparticles
description High-throughput protein microarrays have advanced scientific study into previously very complicated fields such as identification of diseases related proteins and their respective functions. However, they are still not as successful as DNA microarrays in terms of practical usage in medical field. One of the many reasons is due to low detection signal from microarrays. Hence, they are incapable of producing high quality data from small samples of proteins which is frequent in clinical environment. Hence, this project aim is to improve the fluorescence signal from immunoarrays, a subset of protein microarrays. Two different mechanisms, Surface Plasmon Resonance and Enlarged Receptor Surface, were explored to improve fluorescence signal of immunoarrays. For these mechanisms, gold nanoparticles and silica nanoparticles were needed and they were synthesized by Turkevich method and Stober method respectively. Both mechanisms successfully enhanced fluorescence signals. Surface Enlarged receptor method with silica nanoparticles immobilized on microarray substrate enhanced fluorescence signal 7.3 times. On the other hand, silica coated gold nanoparticles enhanced fluorescence signal by 2.75 times in solution. By no means, these are the best possible enhancements as parameters affecting both mechanisms can be optimized further to produce higher fluorescence signal which will enable detection of protein samples of low concentration.
author2 Li Changming
author_facet Li Changming
Ramoo, Jeevaneswaran
format Final Year Project
author Ramoo, Jeevaneswaran
author_sort Ramoo, Jeevaneswaran
title Fluorescence enhancement of immunoarrays by gold and silica nanoparticles
title_short Fluorescence enhancement of immunoarrays by gold and silica nanoparticles
title_full Fluorescence enhancement of immunoarrays by gold and silica nanoparticles
title_fullStr Fluorescence enhancement of immunoarrays by gold and silica nanoparticles
title_full_unstemmed Fluorescence enhancement of immunoarrays by gold and silica nanoparticles
title_sort fluorescence enhancement of immunoarrays by gold and silica nanoparticles
publishDate 2010
url http://hdl.handle.net/10356/40169
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