Proteomics studies of microbial enzymes of Trichoderma reesei used in the production of renewable energy.

• Main Author: Lim, Tze Chao.
• Other Authors: Sze Siu Kwan
• Format: Final Year Project
• Language: English
• Published: 2010
• Subjects: DRNTU::Science::Biological sciences::Microbiology
• Online Access: http://hdl.handle.net/10356/41842
• Institution: Nanyang Technological University

The global warming, environmental pollution, diminishing oil reservoirs and increased energy demand opened an opportunity to investigate on lignocelluloses biomass conversion to biofuels such as ethanol and other forms of bioenergy. The secretome with lignocelluloses hydrolyzing enzymes by two strains of Trichoderma reesei grown on cellulose, starch and agricultural waste such as saw dust and corn stover were analyzed using a proteomics approach with high throughput isobaric tag for relative and absolute quantification (iTRAQ) technique using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The iTRAQ quantification of the secretory proteins of T. reesei showed significant up-regulation of different cellulose hydrolyzing endocellulases, exocellulases, β-glucosidases and glycoside hydrolases and hemicellulases including endo-1,4-beta-xylanase, beta-xylosidase, alpha-glucuronidase, alpha-L-arabinofuranosidase, beta-mannanase, beta-mannosidases polygalacturonase, glucuronidases and arabinosidases. The lignin degrading enzymes (peroxidases, laccases, glyoxal oxidases, catalase, cytochrome-c oxidase, and superoxide dismutase), proteases, protein translocating transporter proteins and several novel proteins were also identified in the secretome.