Cell adhesion behavior on molecularly engineered surfaces

Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute. Better understanding of biophysical responses of adherent cells is of great importance in optimizing biomaterial surface for tissue engineering applications. In this work, poly(lacti...

Full description

Saved in:
Bibliographic Details
Main Author: Cai, Ning
Other Authors: Liao Kin
Format: Theses and Dissertations
Language:English
Published: 2010
Subjects:
Online Access:https://hdl.handle.net/10356/42229
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-42229
record_format dspace
spelling sg-ntu-dr.10356-422292023-03-03T16:05:56Z Cell adhesion behavior on molecularly engineered surfaces Cai, Ning Liao Kin School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute. Better understanding of biophysical responses of adherent cells is of great importance in optimizing biomaterial surface for tissue engineering applications. In this work, poly(lactic acid) (PLA) and carbon nanotubes (CNT), were surface-modified with extracellular matrix (ECM) proteins to improve their compatibility. The results of adhesion dynamics of porcine esophageal fibroblasts (PEFs) on ECM protein-immobilized PLA and CNT monolayer demonstrate the different biophysical responses of PEFs on unmodified and ECM protein-modified biomaterial surfaces. ECM protein immobilization effectively promotes adhesion of PEFs to biomaterials surfaces. In addition, it is found in the study of temporal effect of functional blocking of beta(1) integrin on cell adhesion strength that functional blocking of beta(1) integrin impaires cell-fibronectin interactions. Adhesion strength is tightly correlated to focal adhesion density, which is explained by peeling and fracture models. Furthermore, Weibull distribution is proved to satisfactorily describe cell adhesion strength in adherent cell population. Useful information about the development of cell adhesion is obtained through Weibull statistical analysis of adhesion strength data. DOCTOR OF PHILOSOPHY (SCBE) 2010-10-04T04:39:30Z 2010-10-04T04:39:30Z 2010 2010 Thesis Cai, N. (2010). Cell adhesion behavior on molecularly engineered surfaces. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/42229 10.32657/10356/42229 en 165 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Cai, Ning
Cell adhesion behavior on molecularly engineered surfaces
description Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute. Better understanding of biophysical responses of adherent cells is of great importance in optimizing biomaterial surface for tissue engineering applications. In this work, poly(lactic acid) (PLA) and carbon nanotubes (CNT), were surface-modified with extracellular matrix (ECM) proteins to improve their compatibility. The results of adhesion dynamics of porcine esophageal fibroblasts (PEFs) on ECM protein-immobilized PLA and CNT monolayer demonstrate the different biophysical responses of PEFs on unmodified and ECM protein-modified biomaterial surfaces. ECM protein immobilization effectively promotes adhesion of PEFs to biomaterials surfaces. In addition, it is found in the study of temporal effect of functional blocking of beta(1) integrin on cell adhesion strength that functional blocking of beta(1) integrin impaires cell-fibronectin interactions. Adhesion strength is tightly correlated to focal adhesion density, which is explained by peeling and fracture models. Furthermore, Weibull distribution is proved to satisfactorily describe cell adhesion strength in adherent cell population. Useful information about the development of cell adhesion is obtained through Weibull statistical analysis of adhesion strength data.
author2 Liao Kin
author_facet Liao Kin
Cai, Ning
format Theses and Dissertations
author Cai, Ning
author_sort Cai, Ning
title Cell adhesion behavior on molecularly engineered surfaces
title_short Cell adhesion behavior on molecularly engineered surfaces
title_full Cell adhesion behavior on molecularly engineered surfaces
title_fullStr Cell adhesion behavior on molecularly engineered surfaces
title_full_unstemmed Cell adhesion behavior on molecularly engineered surfaces
title_sort cell adhesion behavior on molecularly engineered surfaces
publishDate 2010
url https://hdl.handle.net/10356/42229
_version_ 1759857007975727104