Cell adhesion behavior on molecularly engineered surfaces
Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute. Better understanding of biophysical responses of adherent cells is of great importance in optimizing biomaterial surface for tissue engineering applications. In this work, poly(lacti...
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sg-ntu-dr.10356-422292023-03-03T16:05:56Z Cell adhesion behavior on molecularly engineered surfaces Cai, Ning Liao Kin School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute. Better understanding of biophysical responses of adherent cells is of great importance in optimizing biomaterial surface for tissue engineering applications. In this work, poly(lactic acid) (PLA) and carbon nanotubes (CNT), were surface-modified with extracellular matrix (ECM) proteins to improve their compatibility. The results of adhesion dynamics of porcine esophageal fibroblasts (PEFs) on ECM protein-immobilized PLA and CNT monolayer demonstrate the different biophysical responses of PEFs on unmodified and ECM protein-modified biomaterial surfaces. ECM protein immobilization effectively promotes adhesion of PEFs to biomaterials surfaces. In addition, it is found in the study of temporal effect of functional blocking of beta(1) integrin on cell adhesion strength that functional blocking of beta(1) integrin impaires cell-fibronectin interactions. Adhesion strength is tightly correlated to focal adhesion density, which is explained by peeling and fracture models. Furthermore, Weibull distribution is proved to satisfactorily describe cell adhesion strength in adherent cell population. Useful information about the development of cell adhesion is obtained through Weibull statistical analysis of adhesion strength data. DOCTOR OF PHILOSOPHY (SCBE) 2010-10-04T04:39:30Z 2010-10-04T04:39:30Z 2010 2010 Thesis Cai, N. (2010). Cell adhesion behavior on molecularly engineered surfaces. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/42229 10.32657/10356/42229 en 165 p. application/pdf |
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DRNTU::Engineering::Bioengineering Cai, Ning Cell adhesion behavior on molecularly engineered surfaces |
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Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute. Better understanding of biophysical responses of adherent cells is of great importance in optimizing biomaterial surface for tissue engineering applications. In this work, poly(lactic acid) (PLA) and carbon nanotubes (CNT), were surface-modified with extracellular matrix (ECM) proteins to improve their compatibility. The results of adhesion dynamics of porcine esophageal fibroblasts (PEFs) on ECM protein-immobilized PLA and CNT monolayer demonstrate the different biophysical responses of PEFs on unmodified and ECM protein-modified biomaterial surfaces. ECM protein immobilization effectively promotes adhesion of PEFs to biomaterials surfaces. In addition, it is found in the study of temporal effect of functional blocking of beta(1) integrin on cell adhesion strength that functional blocking of beta(1) integrin impaires cell-fibronectin interactions. Adhesion strength is tightly correlated to focal adhesion density, which is explained by peeling and fracture models. Furthermore, Weibull distribution is proved to satisfactorily describe cell adhesion strength in adherent cell population. Useful information about the development of cell adhesion is obtained through Weibull statistical analysis of adhesion strength data. |
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Liao Kin |
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Liao Kin Cai, Ning |
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Theses and Dissertations |
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Cai, Ning |
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Cai, Ning |
title |
Cell adhesion behavior on molecularly engineered surfaces |
title_short |
Cell adhesion behavior on molecularly engineered surfaces |
title_full |
Cell adhesion behavior on molecularly engineered surfaces |
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Cell adhesion behavior on molecularly engineered surfaces |
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Cell adhesion behavior on molecularly engineered surfaces |
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cell adhesion behavior on molecularly engineered surfaces |
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2010 |
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https://hdl.handle.net/10356/42229 |
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1759857007975727104 |