Design and development of tissue engineering scaffolds for the regeneration of human tissues and organs via the integration of advanced computer-based medical imaging and engineering technologies

• Main Authors: Chua, Chee Kai., Leong, Kah Fai., Margram Chandrasekaran.
• Other Authors: School of Mechanical and Aerospace Engineering
• Format: Research Report
• Language: English
• Published: 2010
• Subjects: DRNTU::Engineering::Materials::Biomaterials; DRNTU::Science::Medicine::Tissue engineering
• Online Access: http://hdl.handle.net/10356/42260
• Institution: Nanyang Technological University

In vitro cell culture experiments with Human Primary Osteogenic Sarcoma cells (SaOS-2) cells were performed on the RP scaffolds. Results showed that cell proliferation was more favourable in a 3-dimensinal culture than on a monolayer one. The cells migrated into the inner part of the scaffolds and not just proliferate only on the scaffold surface. It showed that the interconnected porous network achieved by the SLS process was sufficient to induce cell migration, as well as oxygen and nutrient transport, into the inner part of the scaffold. Saos-2 cells were found to prefer PVA/HA scaffolds rather than the pure PVA ones. The incorporation of HA was found to enhance cell attachment, proliferation and distribution of cells in the scaffold. A customized direct perfusion bioreactor for the cultivation of compliant scaffold was developed successfully. Significantly higher cell density was recorded in direct perfusion cultured scaffolds compared to the static cultured ones. The combination of a RPfabricated collagen scaffold and the direct perfusion bioreactor has shown to be able to maximize the proliferation of cells in scaffold and enhance homogeneous distribution of cells. These favourable findings ascertained the feasibility of PVA/HA biocomposite to be processed in SLS without generating any adverse effect for mammalian cell culture. This study contributed to the scientific knowledge that sintered synthetic biomaterials, such as PVA, were able to provide a living environment for cells and support the cell growth.