Nanoelectronic biosensing of dynamic cellular activities
Detecting dynamic cellular activities such as exocytosis and bioelectricity is important in the field of molecular biology and medicine. Comparing to conventional biochemical assay, biophysical measurements, and optical methods, electronic biosensing based on nanomaterials have shown promising platf...
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sg-ntu-dr.10356-428862023-03-03T16:06:26Z Nanoelectronic biosensing of dynamic cellular activities Pui, Tze Sian Ajay Agarwa Chen Peng School of Chemical and Biomedical Engineering DRNTU::Engineering::Nanotechnology Detecting dynamic cellular activities such as exocytosis and bioelectricity is important in the field of molecular biology and medicine. Comparing to conventional biochemical assay, biophysical measurements, and optical methods, electronic biosensing based on nanomaterials have shown promising platform in resolving rapid biological events with ultra sensitivity, high spatial and temporal resolution. Here, we use silicon nanowire (SiNW) and single walled carbon nanotube (SWNT) configured as field effect transistor to detect dynamic cellular activities, in particular the time course of cytokines secreted from immune cells or adipose cells and bioelectrical activities of ion channels from electrogenic cells. In this thesis, perfectly aligned arrays of long SiNWs were fabricated using top-down CMOS (complementary metal oxide semiconductor) compatible fabrication techniques and standardized in large-scale production of microelectronics. We demonstrate that these nanowire devices are able to detect cytokines secreted from cells with femtomolar sensitivity, high specificity, wide detection range, and ability for parallel multiplexed monitoring. The detection platform provide a novel tool to reveal the poorly understood signaling mechanisms of the molecules as well as their relevance in secretion related disease. DOCTOR OF PHILOSOPHY (SCBE) 2011-01-28T08:37:53Z 2011-01-28T08:37:53Z 2011 2011 Thesis Pui, T. S. (2011). Nanoelectronic biosensing of dynamic cellular activities. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/42886 10.32657/10356/42886 en 122 p. application/pdf |
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DRNTU::Engineering::Nanotechnology Pui, Tze Sian Nanoelectronic biosensing of dynamic cellular activities |
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Detecting dynamic cellular activities such as exocytosis and bioelectricity is important in the field of molecular biology and medicine. Comparing to conventional biochemical assay, biophysical measurements, and optical methods, electronic biosensing based on nanomaterials have shown promising platform in resolving rapid biological events with ultra sensitivity, high spatial and temporal resolution. Here, we use silicon nanowire (SiNW) and single walled carbon nanotube (SWNT) configured as field effect transistor to detect dynamic cellular activities, in particular the time course of cytokines secreted from immune cells or adipose cells and bioelectrical activities of ion channels from electrogenic cells. In this thesis, perfectly aligned arrays of long SiNWs were fabricated using top-down CMOS (complementary metal oxide semiconductor) compatible fabrication techniques and standardized in large-scale production of microelectronics. We demonstrate that these nanowire devices are able to detect cytokines secreted from cells with femtomolar sensitivity, high specificity, wide detection range, and ability for parallel multiplexed monitoring. The detection platform provide a novel tool to reveal the poorly understood signaling mechanisms of the molecules as well as their relevance in secretion related disease. |
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Ajay Agarwa |
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Ajay Agarwa Pui, Tze Sian |
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Theses and Dissertations |
author |
Pui, Tze Sian |
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Pui, Tze Sian |
title |
Nanoelectronic biosensing of dynamic cellular activities |
title_short |
Nanoelectronic biosensing of dynamic cellular activities |
title_full |
Nanoelectronic biosensing of dynamic cellular activities |
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Nanoelectronic biosensing of dynamic cellular activities |
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Nanoelectronic biosensing of dynamic cellular activities |
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nanoelectronic biosensing of dynamic cellular activities |
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2011 |
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https://hdl.handle.net/10356/42886 |
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1759857218151251968 |