Characterization of low pathogenic avian influenza viruses subtype H5 isolated in Singapore and RNA interference as an antiviral strategy.

Low pathogenic avian influenza viruses (LPAIVs) have the potential to mutate into high pathogenic viruses, and transmission from avian to human host has been documented. We have characterized LPAIVs H5 subtype isolated in Singapore. Molecular sequence analyses revealed that the LPAIVs contained main...

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Main Author: Yeo, Dawn Su Yin.
Other Authors: Richard J Sugrue
Format: Theses and Dissertations
Language:English
Published: 2011
Subjects:
Online Access:http://hdl.handle.net/10356/43686
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-436862023-02-28T18:46:34Z Characterization of low pathogenic avian influenza viruses subtype H5 isolated in Singapore and RNA interference as an antiviral strategy. Yeo, Dawn Su Yin. Richard J Sugrue School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology Low pathogenic avian influenza viruses (LPAIVs) have the potential to mutate into high pathogenic viruses, and transmission from avian to human host has been documented. We have characterized LPAIVs H5 subtype isolated in Singapore. Molecular sequence analyses revealed that the LPAIVs contained mainly avian signatures and no virulence-associated signatures. We examined the replication and growth characteristics of the LPAIVs in avian, canine and human cells, as well as in a mouse model of infection. Host responses to LPAIV infection were profiled and compared using microarrays and we found that the host gene expression was unique to each virus in each cell line, with the strongest antiviral response to the LPAIVs observed in human cells. Finally, we explored RNA interference as an anti-influenza strategy and developed several cell-based assays to rapidly evaluate custom-designed short-interfering (si)RNAs. Doctor of Philosophy (SBS) 2011-04-20T03:08:13Z 2011-04-20T03:08:13Z 2010 2010 Thesis http://hdl.handle.net/10356/43686 en 238 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Virology
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Virology
Yeo, Dawn Su Yin.
Characterization of low pathogenic avian influenza viruses subtype H5 isolated in Singapore and RNA interference as an antiviral strategy.
description Low pathogenic avian influenza viruses (LPAIVs) have the potential to mutate into high pathogenic viruses, and transmission from avian to human host has been documented. We have characterized LPAIVs H5 subtype isolated in Singapore. Molecular sequence analyses revealed that the LPAIVs contained mainly avian signatures and no virulence-associated signatures. We examined the replication and growth characteristics of the LPAIVs in avian, canine and human cells, as well as in a mouse model of infection. Host responses to LPAIV infection were profiled and compared using microarrays and we found that the host gene expression was unique to each virus in each cell line, with the strongest antiviral response to the LPAIVs observed in human cells. Finally, we explored RNA interference as an anti-influenza strategy and developed several cell-based assays to rapidly evaluate custom-designed short-interfering (si)RNAs.
author2 Richard J Sugrue
author_facet Richard J Sugrue
Yeo, Dawn Su Yin.
format Theses and Dissertations
author Yeo, Dawn Su Yin.
author_sort Yeo, Dawn Su Yin.
title Characterization of low pathogenic avian influenza viruses subtype H5 isolated in Singapore and RNA interference as an antiviral strategy.
title_short Characterization of low pathogenic avian influenza viruses subtype H5 isolated in Singapore and RNA interference as an antiviral strategy.
title_full Characterization of low pathogenic avian influenza viruses subtype H5 isolated in Singapore and RNA interference as an antiviral strategy.
title_fullStr Characterization of low pathogenic avian influenza viruses subtype H5 isolated in Singapore and RNA interference as an antiviral strategy.
title_full_unstemmed Characterization of low pathogenic avian influenza viruses subtype H5 isolated in Singapore and RNA interference as an antiviral strategy.
title_sort characterization of low pathogenic avian influenza viruses subtype h5 isolated in singapore and rna interference as an antiviral strategy.
publishDate 2011
url http://hdl.handle.net/10356/43686
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