Reproducibility and repeatability investigation of localised surface plasmon resonance (LSPR) biochips

• Main Author: Lim, Philicia En Chze.
• Other Authors: Lam Yeng Ming
• Format: Final Year Project
• Language: English
• Published: 2011
• Subjects: DRNTU::Engineering::Materials::Biomaterials; DRNTU::Science::Medicine::Biosensors
• Online Access: http://hdl.handle.net/10356/43728
• Institution: Nanyang Technological University

Localised surface plasmon resonance (LSPR) is a quantitative adsorption measurement of nanosized noble-metallic structure on transparent substrate. In this thesis, quartz and glass are used but quartz is identified as the preferred substrate material. Similar to Surface Plasmon, LSPR is sensitive to the refractive index change of the surrounding environment, which makes it a good transducer to be used in sensing applications. Gold is used in this project due to its better optical and biocompatible properties and chemical stability. Nanosphere lithography (NSL) is an inexpensive and simple technique that exploits the nanoparticles’ ability to self-assemble to produce nanopatterns. The formation of well-densed nanoholes across large areas by NSL is suitable for bio sensing applications, an area of interest in this project. Prostate-specific antigen (PSA) is used for testing of prostate cancer (PCa) in humans due to its higher level of concentration in infected patients. The testing requires both LSPR and fluorescence detection, of which a 647 nm excitation-wavelength fluorescence dye is used in this project.