Electrospinning a small diameter tubular scaffold for tissue engineering blood vessels

The function of scaffolds in tissue engineering is to guide the organization, growth and differentiation of cells in constructs. They should be able to mimic the human artery biologically and mechanically. In this work, the aim was to fabricate a bilayered nanofibrous scaffold (I.D<6mm) using th...

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Main Author: Tay, Kulapattra Chongwen.
Other Authors: School of Materials Science and Engineering
Format: Final Year Project
Language:English
Published: 2011
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Online Access:http://hdl.handle.net/10356/44054
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-440542023-03-04T15:35:52Z Electrospinning a small diameter tubular scaffold for tissue engineering blood vessels Tay, Kulapattra Chongwen. School of Materials Science and Engineering Ng Kee Woei DRNTU::Engineering::Materials::Biomaterials The function of scaffolds in tissue engineering is to guide the organization, growth and differentiation of cells in constructs. They should be able to mimic the human artery biologically and mechanically. In this work, the aim was to fabricate a bilayered nanofibrous scaffold (I.D<6mm) using the electrospinning technique. The first layer would be aligned longitudinally and the second layer aligned circumferentially. This alignment mimics the respective directions of the endothelial cells and smooth muscle cells in the human blood vessel. A variety of parameters were first experimented to achieve the best circumferential and longitudinal configurations, before electrospinning a bilayer scaffold. The biodegradable material used was Poly(L-lactide-ϵ-caprolactone) (PLC). The scaffold was also subjected to characterization techniques using Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA) and Fourier-Transform Infrared Spectroscopy (FTIR) to determine changes in the intrinsic properties of PLC before and after electrospinning. In this study, a longitudinally aligned nanofibrous scaffold with an inner diameter of 3.2mm and wall thickness of 70.3µm was fabricated. However a circumferentially alignment was not produced. The maximum rotation speed (2000 rpm) that the stirrer machine could provide was believed to be insufficient to attain a circumferential alignment on the tube. The bilayered tubular scaffold was still developed, but with an aligned inner layer and a random outer layer. The results from DSC, TGA and FTIR showed no large deviation in thermal and chemical properties of PLC before and after electrospinning. Bachelor of Engineering (Materials Engineering) 2011-05-20T08:25:47Z 2011-05-20T08:25:47Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/44054 en Nanyang Technological University 47 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Tay, Kulapattra Chongwen.
Electrospinning a small diameter tubular scaffold for tissue engineering blood vessels
description The function of scaffolds in tissue engineering is to guide the organization, growth and differentiation of cells in constructs. They should be able to mimic the human artery biologically and mechanically. In this work, the aim was to fabricate a bilayered nanofibrous scaffold (I.D<6mm) using the electrospinning technique. The first layer would be aligned longitudinally and the second layer aligned circumferentially. This alignment mimics the respective directions of the endothelial cells and smooth muscle cells in the human blood vessel. A variety of parameters were first experimented to achieve the best circumferential and longitudinal configurations, before electrospinning a bilayer scaffold. The biodegradable material used was Poly(L-lactide-ϵ-caprolactone) (PLC). The scaffold was also subjected to characterization techniques using Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA) and Fourier-Transform Infrared Spectroscopy (FTIR) to determine changes in the intrinsic properties of PLC before and after electrospinning. In this study, a longitudinally aligned nanofibrous scaffold with an inner diameter of 3.2mm and wall thickness of 70.3µm was fabricated. However a circumferentially alignment was not produced. The maximum rotation speed (2000 rpm) that the stirrer machine could provide was believed to be insufficient to attain a circumferential alignment on the tube. The bilayered tubular scaffold was still developed, but with an aligned inner layer and a random outer layer. The results from DSC, TGA and FTIR showed no large deviation in thermal and chemical properties of PLC before and after electrospinning.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Tay, Kulapattra Chongwen.
format Final Year Project
author Tay, Kulapattra Chongwen.
author_sort Tay, Kulapattra Chongwen.
title Electrospinning a small diameter tubular scaffold for tissue engineering blood vessels
title_short Electrospinning a small diameter tubular scaffold for tissue engineering blood vessels
title_full Electrospinning a small diameter tubular scaffold for tissue engineering blood vessels
title_fullStr Electrospinning a small diameter tubular scaffold for tissue engineering blood vessels
title_full_unstemmed Electrospinning a small diameter tubular scaffold for tissue engineering blood vessels
title_sort electrospinning a small diameter tubular scaffold for tissue engineering blood vessels
publishDate 2011
url http://hdl.handle.net/10356/44054
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