TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems

Articular degenerative diseases like osteoarthritis significantly affect the life quality of worldwide populations including Singaporean communities. Traditional approaches, typically transplantation of autologous tissue grafts, exhibit many limitations in clinic because of implants resorption and p...

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Main Author: Rohan Rajeev Varshney
Other Authors: Wang Dongan
Format: Theses and Dissertations
Language:English
Published: 2011
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Online Access:https://hdl.handle.net/10356/44582
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-445822023-03-03T16:07:41Z TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems Rohan Rajeev Varshney Wang Dongan School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology DRNTU::Science::Medicine::Tissue engineering Articular degenerative diseases like osteoarthritis significantly affect the life quality of worldwide populations including Singaporean communities. Traditional approaches, typically transplantation of autologous tissue grafts, exhibit many limitations in clinic because of implants resorption and poor shaping with articular defects, as well as lack of sufficient materials donors. In the recent decade, novel strategies with tissue engineering and gene therapy have been developed from laboratory-based investigation to clinical applications. With the development of gene delivery to chondrocytic/progenitor therapeutic cells such as mesenchymal stem cells, treating disorders of articular cartilage by tissue engineering has borne much promise. Mesenchymal stem cells (MSCs) have been proven to be efficacious in articular cartilage repair and have even been used in a few in vivo clinical trials. Recently the focus has extended to synovium-derived MSCs (SMSCs) owing to their excellent chondrogenic potential. These cells are highly proliferative and can be expanded to sufficiently large numbers. Research has been carried out to induce chondrogenesis and engineer cartilaginous constructs from SMSCs using gene therapy and tissue engineering, with some degree of success. This study focuses on SMSCs and transgenic chondrocytes based co-culture system for engineered chondrogenesis. DOCTOR OF PHILOSOPHY (SCBE) 2011-06-02T07:07:51Z 2011-06-02T07:07:51Z 2011 2011 Thesis Rohan, R. V. (2011). TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/44582 10.32657/10356/44582 en 172 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biotechnology
DRNTU::Science::Medicine::Tissue engineering
spellingShingle DRNTU::Engineering::Chemical engineering::Biotechnology
DRNTU::Science::Medicine::Tissue engineering
Rohan Rajeev Varshney
TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems
description Articular degenerative diseases like osteoarthritis significantly affect the life quality of worldwide populations including Singaporean communities. Traditional approaches, typically transplantation of autologous tissue grafts, exhibit many limitations in clinic because of implants resorption and poor shaping with articular defects, as well as lack of sufficient materials donors. In the recent decade, novel strategies with tissue engineering and gene therapy have been developed from laboratory-based investigation to clinical applications. With the development of gene delivery to chondrocytic/progenitor therapeutic cells such as mesenchymal stem cells, treating disorders of articular cartilage by tissue engineering has borne much promise. Mesenchymal stem cells (MSCs) have been proven to be efficacious in articular cartilage repair and have even been used in a few in vivo clinical trials. Recently the focus has extended to synovium-derived MSCs (SMSCs) owing to their excellent chondrogenic potential. These cells are highly proliferative and can be expanded to sufficiently large numbers. Research has been carried out to induce chondrogenesis and engineer cartilaginous constructs from SMSCs using gene therapy and tissue engineering, with some degree of success. This study focuses on SMSCs and transgenic chondrocytes based co-culture system for engineered chondrogenesis.
author2 Wang Dongan
author_facet Wang Dongan
Rohan Rajeev Varshney
format Theses and Dissertations
author Rohan Rajeev Varshney
author_sort Rohan Rajeev Varshney
title TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems
title_short TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems
title_full TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems
title_fullStr TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems
title_full_unstemmed TGF-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems
title_sort tgf-β3 mediated chondrogenic differentiation of synovial mesenchymal stem cells in gene transferred co-culture systems
publishDate 2011
url https://hdl.handle.net/10356/44582
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