Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons.

Pruning, which selectively eliminates superfluous neuronal processes without causing neuronal death, is an important process of neuronal remodeling during development. Here in the first part of this study, we report the results of a genome-wide RNA interference screen in Drosophila melanogaster clas...

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Main Author: Zhou, Zimin.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2011
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Online Access:http://hdl.handle.net/10356/44651
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-446512023-02-28T18:04:36Z Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons. Zhou, Zimin. School of Biological Sciences Temasek Laboratories Yu FengWei DRNTU::Science Pruning, which selectively eliminates superfluous neuronal processes without causing neuronal death, is an important process of neuronal remodeling during development. Here in the first part of this study, we report the results of a genome-wide RNA interference screen in Drosophila melanogaster class IV dendritic arborization neurons (ddaC) for novel genes that are involved in dendrite pruning. We identified four novel genes: Nmnat (Nicotinamide mononucleotide adenylyltransferase); Snap (Soluble NSF attachment protein); Ank2 (Ankyrin 2); Msps (Mini Spindles). Knocking-down these four genes by RNAi result in dendrite pruning defects in ddaC neurons at 16h after puparium formation (16h APF). In the second part of this study, we used double RNAi screen to identify other molecules that may be related to Mical (Molecule interacting with casL) during pruning process. We isolated 12 genes that may function parallel to or synergistically with Mical, as well as one gene called Unc-115 (Uncoordinated 115) that may operate in the same genetic pathway with Mical. Therefore, our findings have enlarged the number of known genes that affect pruning process and will provide insights into cellular and molecular mechanisms of neuronal pruning. Bachelor of Science in Biological Sciences 2011-06-03T01:02:50Z 2011-06-03T01:02:50Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/44651 en Nanyang Technological University 39 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Zhou, Zimin.
Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons.
description Pruning, which selectively eliminates superfluous neuronal processes without causing neuronal death, is an important process of neuronal remodeling during development. Here in the first part of this study, we report the results of a genome-wide RNA interference screen in Drosophila melanogaster class IV dendritic arborization neurons (ddaC) for novel genes that are involved in dendrite pruning. We identified four novel genes: Nmnat (Nicotinamide mononucleotide adenylyltransferase); Snap (Soluble NSF attachment protein); Ank2 (Ankyrin 2); Msps (Mini Spindles). Knocking-down these four genes by RNAi result in dendrite pruning defects in ddaC neurons at 16h after puparium formation (16h APF). In the second part of this study, we used double RNAi screen to identify other molecules that may be related to Mical (Molecule interacting with casL) during pruning process. We isolated 12 genes that may function parallel to or synergistically with Mical, as well as one gene called Unc-115 (Uncoordinated 115) that may operate in the same genetic pathway with Mical. Therefore, our findings have enlarged the number of known genes that affect pruning process and will provide insights into cellular and molecular mechanisms of neuronal pruning.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Zhou, Zimin.
format Final Year Project
author Zhou, Zimin.
author_sort Zhou, Zimin.
title Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons.
title_short Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons.
title_full Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons.
title_fullStr Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons.
title_full_unstemmed Cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons.
title_sort cellular and molecular mechanisms of neuronal pruning in dendrite arborization neurons.
publishDate 2011
url http://hdl.handle.net/10356/44651
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