The involvement of SPARC and HEVIN in angiogenesis in the retina.

Angiogenesis is the formation of new blood vessels from existing ones and is regulated by a balance of pro- and anti-angiogenic factors such as vascular endothelial growth factors and platelet-derived growth factor. An imbalance will result in the activation of unwanted angiogenesis which can occur...

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Main Author: Goh, Carine Chun Ai.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2011
Subjects:
Online Access:http://hdl.handle.net/10356/45122
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-451222023-02-28T18:01:58Z The involvement of SPARC and HEVIN in angiogenesis in the retina. Goh, Carine Chun Ai. School of Biological Sciences Seet Li Fong Tina Wong DRNTU::Science::Biological sciences::Molecular biology Angiogenesis is the formation of new blood vessels from existing ones and is regulated by a balance of pro- and anti-angiogenic factors such as vascular endothelial growth factors and platelet-derived growth factor. An imbalance will result in the activation of unwanted angiogenesis which can occur in a range of ocular diseases such as age-related macular degeneration and diabetic retinopathy. Matricellular proteins like SPARC and HEVIN are gaining interest for their role in the regulation of cellular functions as well as angiogenesis. The role of SPARC in angiogenesis has been studied extensively, but not in relation to the retinal pigment epithelium in retina. HEVIN, a homologue of SPARC, may regulate angiogenesis in a similar manner. To better understand the mechanisms underlying SPARC and HEVIN mediated inhibition of angiogenesis in the retina, we carried out a RT2 Profiler PCR Array to profile the expression of 84 genes related to mouse angiogenesis signaling pathway with RPE cells isolated from mice depleted with SPARC and HEVIN. The findings of our study revealed that both SPARC and HEVIN regulate the expression of collagen, VEGFs, PDGF, MMP2 and MMP9 in angiogenesis but they may not necessary function in a similar mechanism. Bachelor of Science in Biological Sciences 2011-06-09T03:59:33Z 2011-06-09T03:59:33Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45122 en Nanyang Technological University 40 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Molecular biology
spellingShingle DRNTU::Science::Biological sciences::Molecular biology
Goh, Carine Chun Ai.
The involvement of SPARC and HEVIN in angiogenesis in the retina.
description Angiogenesis is the formation of new blood vessels from existing ones and is regulated by a balance of pro- and anti-angiogenic factors such as vascular endothelial growth factors and platelet-derived growth factor. An imbalance will result in the activation of unwanted angiogenesis which can occur in a range of ocular diseases such as age-related macular degeneration and diabetic retinopathy. Matricellular proteins like SPARC and HEVIN are gaining interest for their role in the regulation of cellular functions as well as angiogenesis. The role of SPARC in angiogenesis has been studied extensively, but not in relation to the retinal pigment epithelium in retina. HEVIN, a homologue of SPARC, may regulate angiogenesis in a similar manner. To better understand the mechanisms underlying SPARC and HEVIN mediated inhibition of angiogenesis in the retina, we carried out a RT2 Profiler PCR Array to profile the expression of 84 genes related to mouse angiogenesis signaling pathway with RPE cells isolated from mice depleted with SPARC and HEVIN. The findings of our study revealed that both SPARC and HEVIN regulate the expression of collagen, VEGFs, PDGF, MMP2 and MMP9 in angiogenesis but they may not necessary function in a similar mechanism.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Goh, Carine Chun Ai.
format Final Year Project
author Goh, Carine Chun Ai.
author_sort Goh, Carine Chun Ai.
title The involvement of SPARC and HEVIN in angiogenesis in the retina.
title_short The involvement of SPARC and HEVIN in angiogenesis in the retina.
title_full The involvement of SPARC and HEVIN in angiogenesis in the retina.
title_fullStr The involvement of SPARC and HEVIN in angiogenesis in the retina.
title_full_unstemmed The involvement of SPARC and HEVIN in angiogenesis in the retina.
title_sort involvement of sparc and hevin in angiogenesis in the retina.
publishDate 2011
url http://hdl.handle.net/10356/45122
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