Novel method for protein microencapsulation in polyelectrolyte microparticles.

Tremendous advancements in proteomics have aroused great interest in the development of protein therapeutics for human ailments, thereby increasing the demand for innovative, safe and efficient protein delivery methods. Polyelectrolyte microparticles (PEMP) represent a novel type of highly customiza...

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Main Author: Sharon Harvinder Kaur Dhillon.
Other Authors: Bjoern Holger Neu
Format: Final Year Project
Language:English
Published: 2011
Subjects:
Online Access:http://hdl.handle.net/10356/45281
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-452812023-03-03T15:35:20Z Novel method for protein microencapsulation in polyelectrolyte microparticles. Sharon Harvinder Kaur Dhillon. Bjoern Holger Neu School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology Tremendous advancements in proteomics have aroused great interest in the development of protein therapeutics for human ailments, thereby increasing the demand for innovative, safe and efficient protein delivery methods. Polyelectrolyte microparticles (PEMP) represent a novel type of highly customizable carrier system for the delivery of bioactive therapeutics. PEMP are fabricated by the layer-by-layer (LbL) adsorption of oppositely charged polyelectrolytes onto a colloidal template and can be customized by varying properties such as multilayer composition and colloidal template used. This study aims to investigate a novel microencapsulation technique via the incorporation of proteins as a layer constituent within polyelectrolyte multilayers, to ultimately fabricate PEMP for protein delivery purposes. Biodegradable and biocompatible PEMP were formed by the LbL adsorption of PRM/DXS polyelectrolytes onto a CaCO3 colloidal core. Model proteins lysozyme and BSA were separately adsorbed within PRM/DXS multilayer shells and the formation and stability of protein-loaded multilayers was confirmed using confocal microscopy, flow cytometry and zeta potential measurements. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2011-06-10T07:03:44Z 2011-06-10T07:03:44Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45281 en Nanyang Technological University 80 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biotechnology
spellingShingle DRNTU::Engineering::Chemical engineering::Biotechnology
Sharon Harvinder Kaur Dhillon.
Novel method for protein microencapsulation in polyelectrolyte microparticles.
description Tremendous advancements in proteomics have aroused great interest in the development of protein therapeutics for human ailments, thereby increasing the demand for innovative, safe and efficient protein delivery methods. Polyelectrolyte microparticles (PEMP) represent a novel type of highly customizable carrier system for the delivery of bioactive therapeutics. PEMP are fabricated by the layer-by-layer (LbL) adsorption of oppositely charged polyelectrolytes onto a colloidal template and can be customized by varying properties such as multilayer composition and colloidal template used. This study aims to investigate a novel microencapsulation technique via the incorporation of proteins as a layer constituent within polyelectrolyte multilayers, to ultimately fabricate PEMP for protein delivery purposes. Biodegradable and biocompatible PEMP were formed by the LbL adsorption of PRM/DXS polyelectrolytes onto a CaCO3 colloidal core. Model proteins lysozyme and BSA were separately adsorbed within PRM/DXS multilayer shells and the formation and stability of protein-loaded multilayers was confirmed using confocal microscopy, flow cytometry and zeta potential measurements.
author2 Bjoern Holger Neu
author_facet Bjoern Holger Neu
Sharon Harvinder Kaur Dhillon.
format Final Year Project
author Sharon Harvinder Kaur Dhillon.
author_sort Sharon Harvinder Kaur Dhillon.
title Novel method for protein microencapsulation in polyelectrolyte microparticles.
title_short Novel method for protein microencapsulation in polyelectrolyte microparticles.
title_full Novel method for protein microencapsulation in polyelectrolyte microparticles.
title_fullStr Novel method for protein microencapsulation in polyelectrolyte microparticles.
title_full_unstemmed Novel method for protein microencapsulation in polyelectrolyte microparticles.
title_sort novel method for protein microencapsulation in polyelectrolyte microparticles.
publishDate 2011
url http://hdl.handle.net/10356/45281
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