Surface properties of red blood cell ghosts

Red blood cells (RBC) are of current basic science and clinical research due to the advantages it offers in the biomedical field. Vesicles derived from RBC have been explored in drug delivery applications due to the non-immunogenic, biocompatible and biodegradable properties. In this project, two ty...

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Main Author: Gan, Yan Li
Other Authors: Bjoern Holger Neu
Format: Final Year Project
Language:English
Published: 2011
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Online Access:http://hdl.handle.net/10356/45287
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-452872023-03-03T15:32:02Z Surface properties of red blood cell ghosts Gan, Yan Li Bjoern Holger Neu School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology DRNTU::Science::Chemistry::Physical chemistry::Surface chemistry Red blood cells (RBC) are of current basic science and clinical research due to the advantages it offers in the biomedical field. Vesicles derived from RBC have been explored in drug delivery applications due to the non-immunogenic, biocompatible and biodegradable properties. In this project, two types of RBC ghost vesicles are investigated, namely inside-out vesicles (IOV) and right-side-out vesicles (ROV). IOV have an inverted membrane orientation whereas ROV have the native membrane orientation as compared to the RBC ghosts. The inverted membrane orientation of the IOV is similar to senescent RBC and other apoptotic cells with high number of phosphotidylserine translocated to the outer monolayer. The vesicles were characterized by quantification of surface markers such as sialic acid and phosphotidylserine using flow cytometry. In this work, surface properties of the vesicles were characterized by monitoring the zeta potential at various ionic strength and pH. It was found that ROV is more negatively charged than IOV due to higher amount of sialic acid present on the extracellular surface. Understanding of the composition of surface groups through fingerprinting of the vesicles aids in possible surface modification. By differentiating the surface charges of vesicles, the time for drug release could be controlled and thus enhancing drug delivery targeting of the reticuloendothelial system, especially spleen and liver. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2011-06-10T07:16:22Z 2011-06-10T07:16:22Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45287 en Nanyang Technological University 58 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biotechnology
DRNTU::Science::Chemistry::Physical chemistry::Surface chemistry
spellingShingle DRNTU::Engineering::Chemical engineering::Biotechnology
DRNTU::Science::Chemistry::Physical chemistry::Surface chemistry
Gan, Yan Li
Surface properties of red blood cell ghosts
description Red blood cells (RBC) are of current basic science and clinical research due to the advantages it offers in the biomedical field. Vesicles derived from RBC have been explored in drug delivery applications due to the non-immunogenic, biocompatible and biodegradable properties. In this project, two types of RBC ghost vesicles are investigated, namely inside-out vesicles (IOV) and right-side-out vesicles (ROV). IOV have an inverted membrane orientation whereas ROV have the native membrane orientation as compared to the RBC ghosts. The inverted membrane orientation of the IOV is similar to senescent RBC and other apoptotic cells with high number of phosphotidylserine translocated to the outer monolayer. The vesicles were characterized by quantification of surface markers such as sialic acid and phosphotidylserine using flow cytometry. In this work, surface properties of the vesicles were characterized by monitoring the zeta potential at various ionic strength and pH. It was found that ROV is more negatively charged than IOV due to higher amount of sialic acid present on the extracellular surface. Understanding of the composition of surface groups through fingerprinting of the vesicles aids in possible surface modification. By differentiating the surface charges of vesicles, the time for drug release could be controlled and thus enhancing drug delivery targeting of the reticuloendothelial system, especially spleen and liver.
author2 Bjoern Holger Neu
author_facet Bjoern Holger Neu
Gan, Yan Li
format Final Year Project
author Gan, Yan Li
author_sort Gan, Yan Li
title Surface properties of red blood cell ghosts
title_short Surface properties of red blood cell ghosts
title_full Surface properties of red blood cell ghosts
title_fullStr Surface properties of red blood cell ghosts
title_full_unstemmed Surface properties of red blood cell ghosts
title_sort surface properties of red blood cell ghosts
publishDate 2011
url http://hdl.handle.net/10356/45287
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