In situ selective cellular nitric oxide sensing using humanized graphene film

• Main Author: Khoo, Si Yun.
• Other Authors: Li Changming
• Format: Final Year Project
• Language: English
• Published: 2011
• Subjects: DRNTU::Science::Medicine::Biosensors
• Online Access: http://hdl.handle.net/10356/45596
• Institution: Nanyang Technological University

Nitric oxide is a messenger molecule and plays important roles in maintaining physiological function. The abnormality of NO concentration presence in biological body would lead to pathogenesis. This project reports the construction of RGD-peptide grafted graphene film and its application as a selective and flexible sensing matrix for cell released nitric oxide molecule detection. The constructed flexible graphene sensing matrix is characterized using scanning electron microscope, atomic force microscopic, reflection absorption infrared spectroscopy and proteomic test. The results show that the RGD-peptide is successfully grafted onto –COOH functionalized graphene film. Electrochemical characterizations tell that the graphene sensing matrix provides a sensitivity of 10.718 μA/μM, detection limit of 194 nM, and a broad linear range from 4 μM to 0.8 mM. In addition, the sensing matrix also exhibits good biocompatibility for human cells. More importantly, the sensing matrix has demonstrated its capability for in situ dynamic cell nitric oxide molecular detection. This work provides a unique sensing unique sensing matrix for various cell molecular detections.