Profiling and authentication of Triticum aestivum (浮小麦).
Traditional Chinese Medicine (TCM) and its formulations have a long history and have been prevalently used. However, the active components present are not well understood. Currently, researches done are mainly focusing on small molecules that may have biological activities. In this study, we...
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sg-ntu-dr.10356-462222023-02-28T18:00:49Z Profiling and authentication of Triticum aestivum (浮小麦). Ng, Hui Wen. School of Biological Sciences James P. Tam DRNTU::Science::Biological sciences::Botany::Plant physiology Traditional Chinese Medicine (TCM) and its formulations have a long history and have been prevalently used. However, the active components present are not well understood. Currently, researches done are mainly focusing on small molecules that may have biological activities. In this study, we report the discovery of Cysteine-rich peptides (CRPs) in Triticum aestivum (浮小麦), which is the immature form of wheat used in TCM to treat night sweat. Triticum aestivum from different sources were examined using high performance liquid chromatograpy (HPLC), utilising ultra performance liquid chromatography (UPLC) and mass spectrometry (MS). Our results reveal differences in the quality and constituents of Triticum aestivum from different sources. This suggests that herbal medicine may differ in their efficacy depending on the source. Therefore, these issues highlight the need for proper authentication and quality control in TCM. We proceed to characterize CRPs by UPLC and MS to examine their stability to thermal and enzymatic degradation. Interestingly, the CRPs were found to be relatively resistant and stable. This suggests that CRPs may be putative active ingredients in TCM and can potentially be used for the design of orally active drugs. Bachelor of Science in Biological Sciences 2011-07-08T01:08:35Z 2011-07-08T01:08:35Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/46222 en Nanyang Technological University 47 p. application/pdf |
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DRNTU::Science::Biological sciences::Botany::Plant physiology Ng, Hui Wen. Profiling and authentication of Triticum aestivum (浮小麦). |
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Traditional Chinese Medicine (TCM) and its formulations have a long history and have
been prevalently used. However, the active components present are not well understood. Currently, researches done are mainly focusing on small molecules that may have biological activities. In this study, we report the discovery of Cysteine-rich peptides (CRPs) in Triticum aestivum (浮小麦), which is the immature form of wheat
used in TCM to treat night sweat. Triticum aestivum from different sources were examined using high performance liquid chromatograpy (HPLC), utilising ultra
performance liquid chromatography (UPLC) and mass spectrometry (MS). Our results
reveal differences in the quality and constituents of Triticum aestivum from different sources. This suggests that herbal medicine may differ in their efficacy depending on the source. Therefore, these issues highlight the need for proper authentication and quality control in TCM. We proceed to characterize CRPs by UPLC and MS to examine their stability to thermal and enzymatic degradation. Interestingly, the CRPs were found to be relatively resistant and stable. This suggests that CRPs may be
putative active ingredients in TCM and can potentially be used for the design of orally
active drugs. |
author2 |
School of Biological Sciences |
author_facet |
School of Biological Sciences Ng, Hui Wen. |
format |
Final Year Project |
author |
Ng, Hui Wen. |
author_sort |
Ng, Hui Wen. |
title |
Profiling and authentication of Triticum aestivum (浮小麦). |
title_short |
Profiling and authentication of Triticum aestivum (浮小麦). |
title_full |
Profiling and authentication of Triticum aestivum (浮小麦). |
title_fullStr |
Profiling and authentication of Triticum aestivum (浮小麦). |
title_full_unstemmed |
Profiling and authentication of Triticum aestivum (浮小麦). |
title_sort |
profiling and authentication of triticum aestivum (浮小麦). |
publishDate |
2011 |
url |
http://hdl.handle.net/10356/46222 |
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1759857434470383616 |