Profiling and authentication of Triticum aestivum (浮小麦).

Traditional Chinese Medicine (TCM) and its formulations have a long history and have been prevalently used. However, the active components present are not well understood. Currently, researches done are mainly focusing on small molecules that may have biological activities. In this study, we...

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Main Author: Ng, Hui Wen.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2011
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Online Access:http://hdl.handle.net/10356/46222
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-462222023-02-28T18:00:49Z Profiling and authentication of Triticum aestivum (浮小麦). Ng, Hui Wen. School of Biological Sciences James P. Tam DRNTU::Science::Biological sciences::Botany::Plant physiology Traditional Chinese Medicine (TCM) and its formulations have a long history and have been prevalently used. However, the active components present are not well understood. Currently, researches done are mainly focusing on small molecules that may have biological activities. In this study, we report the discovery of Cysteine-rich peptides (CRPs) in Triticum aestivum (浮小麦), which is the immature form of wheat used in TCM to treat night sweat. Triticum aestivum from different sources were examined using high performance liquid chromatograpy (HPLC), utilising ultra performance liquid chromatography (UPLC) and mass spectrometry (MS). Our results reveal differences in the quality and constituents of Triticum aestivum from different sources. This suggests that herbal medicine may differ in their efficacy depending on the source. Therefore, these issues highlight the need for proper authentication and quality control in TCM. We proceed to characterize CRPs by UPLC and MS to examine their stability to thermal and enzymatic degradation. Interestingly, the CRPs were found to be relatively resistant and stable. This suggests that CRPs may be putative active ingredients in TCM and can potentially be used for the design of orally active drugs. Bachelor of Science in Biological Sciences 2011-07-08T01:08:35Z 2011-07-08T01:08:35Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/46222 en Nanyang Technological University 47 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Botany::Plant physiology
spellingShingle DRNTU::Science::Biological sciences::Botany::Plant physiology
Ng, Hui Wen.
Profiling and authentication of Triticum aestivum (浮小麦).
description Traditional Chinese Medicine (TCM) and its formulations have a long history and have been prevalently used. However, the active components present are not well understood. Currently, researches done are mainly focusing on small molecules that may have biological activities. In this study, we report the discovery of Cysteine-rich peptides (CRPs) in Triticum aestivum (浮小麦), which is the immature form of wheat used in TCM to treat night sweat. Triticum aestivum from different sources were examined using high performance liquid chromatograpy (HPLC), utilising ultra performance liquid chromatography (UPLC) and mass spectrometry (MS). Our results reveal differences in the quality and constituents of Triticum aestivum from different sources. This suggests that herbal medicine may differ in their efficacy depending on the source. Therefore, these issues highlight the need for proper authentication and quality control in TCM. We proceed to characterize CRPs by UPLC and MS to examine their stability to thermal and enzymatic degradation. Interestingly, the CRPs were found to be relatively resistant and stable. This suggests that CRPs may be putative active ingredients in TCM and can potentially be used for the design of orally active drugs.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Ng, Hui Wen.
format Final Year Project
author Ng, Hui Wen.
author_sort Ng, Hui Wen.
title Profiling and authentication of Triticum aestivum (浮小麦).
title_short Profiling and authentication of Triticum aestivum (浮小麦).
title_full Profiling and authentication of Triticum aestivum (浮小麦).
title_fullStr Profiling and authentication of Triticum aestivum (浮小麦).
title_full_unstemmed Profiling and authentication of Triticum aestivum (浮小麦).
title_sort profiling and authentication of triticum aestivum (浮小麦).
publishDate 2011
url http://hdl.handle.net/10356/46222
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