The regulation of the actin cytoskeleton and cell cycle progression

Cell cycle progression in metazoans proceeds through a series of highly regulated events. In vitro, adherent cell types exhibit substratum adherence and a well spread shape during interphase, which collapse during mitosis to form rounded cells with increased cortical rigidity. After mitosis, there i...

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Main Author: Heng, Yi Wen
Other Authors: Koh Cheng Gee
Format: Theses and Dissertations
Language:English
Published: 2011
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Online Access:https://hdl.handle.net/10356/46278
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-462782023-02-28T18:46:36Z The regulation of the actin cytoskeleton and cell cycle progression Heng, Yi Wen Koh Cheng Gee School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology Cell cycle progression in metazoans proceeds through a series of highly regulated events. In vitro, adherent cell types exhibit substratum adherence and a well spread shape during interphase, which collapse during mitosis to form rounded cells with increased cortical rigidity. After mitosis, there is re-establishment of attachment and cytoskeletal tension. Modulation of such drastic changes in cell shape in synchrony to cell cycle progression suggests tight signalling regulation between cytoskeleton dynamics and cell cycle progression. We aim to study the regulatory links between the actin cytoskeleton, its regulators and effectors on the cell cycle progression. We found that perturbation of actin using various drugs were able to alter the timings of progression into various cell cycle stages. In addition, we observed that the RhoA signalling pathway can control metaphase astral microtubules formation through its effector ROCK, LIMK2 and its novel substrate tubulin polymerising promoting protein (TPPP), and thus affect spindle orientation. Finally, we suggest that LIMK1 and LIMK2 can play differential role during mitosis; LIMK1 modulating centrosomes integrity and LIMK2 regulating astral microtubules formation. DOCTOR OF PHILOSOPHY (SBS) 2011-11-28T04:09:24Z 2011-11-28T04:09:24Z 2011 2011 Thesis Heng, Y. W. (2011). The regulation of the actin cytoskeleton and cell cycle progression. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/46278 10.32657/10356/46278 en 174 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Molecular biology
spellingShingle DRNTU::Science::Biological sciences::Molecular biology
Heng, Yi Wen
The regulation of the actin cytoskeleton and cell cycle progression
description Cell cycle progression in metazoans proceeds through a series of highly regulated events. In vitro, adherent cell types exhibit substratum adherence and a well spread shape during interphase, which collapse during mitosis to form rounded cells with increased cortical rigidity. After mitosis, there is re-establishment of attachment and cytoskeletal tension. Modulation of such drastic changes in cell shape in synchrony to cell cycle progression suggests tight signalling regulation between cytoskeleton dynamics and cell cycle progression. We aim to study the regulatory links between the actin cytoskeleton, its regulators and effectors on the cell cycle progression. We found that perturbation of actin using various drugs were able to alter the timings of progression into various cell cycle stages. In addition, we observed that the RhoA signalling pathway can control metaphase astral microtubules formation through its effector ROCK, LIMK2 and its novel substrate tubulin polymerising promoting protein (TPPP), and thus affect spindle orientation. Finally, we suggest that LIMK1 and LIMK2 can play differential role during mitosis; LIMK1 modulating centrosomes integrity and LIMK2 regulating astral microtubules formation.
author2 Koh Cheng Gee
author_facet Koh Cheng Gee
Heng, Yi Wen
format Theses and Dissertations
author Heng, Yi Wen
author_sort Heng, Yi Wen
title The regulation of the actin cytoskeleton and cell cycle progression
title_short The regulation of the actin cytoskeleton and cell cycle progression
title_full The regulation of the actin cytoskeleton and cell cycle progression
title_fullStr The regulation of the actin cytoskeleton and cell cycle progression
title_full_unstemmed The regulation of the actin cytoskeleton and cell cycle progression
title_sort regulation of the actin cytoskeleton and cell cycle progression
publishDate 2011
url https://hdl.handle.net/10356/46278
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