High efficiencies of gene delivery and gene targeting in embryonic stem cells of the fish medaka as a lower vertebrate model

• المؤلف الرئيسي: Yan, Yan
• مؤلفون آخرون: Hong YunHan
• التنسيق: Theses and Dissertations
• اللغة: English
• منشور في: 2011
• الموضوعات: DRNTU::Engineering::Chemical engineering::Biotechnology
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/46530

This study reports the high efficiencies of gene delivery and gene targeting (GT) in a medaka fish embryonic stem (ES) cell model. A baculoviral (BV) vector is demonstrated to significantly improve gene delivery in medaka ES (MES I) cells, Furthermore, the BV mediated host integration does not compr...