Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering

123 p.

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Bibliographic Details
Main Author:	Myint Zu Myaing.
Other Authors:	Chian Kerm Sin
Format:	Theses and Dissertations
Published:	2011
Subjects:	DRNTU::Engineering::Bioengineering
Online Access:	http://hdl.handle.net/10356/47078
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Institution:	Nanyang Technological University

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spelling	sg-ntu-dr.10356-470782023-03-11T17:15:39Z Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering Myint Zu Myaing. Chian Kerm Sin School of Mechanical and Aerospace Engineering DRNTU::Engineering::Bioengineering 123 p. A number of decellularization methods have been developed to produce acellular xenogenic extracellular matrix (ECM) scaffolds to reconstruct oesophageal defects due to either congenital or acquired diseases. However, the effects of decellularization process on the resulted ECMs have not been fully elucidated yet. The aim of this project is to produce acellular porcine oesophageal scaffolds for oesophageal tissue engineering application. In this study, two different decellularization methods were established; (1) a chemical-based process (using peracetic acid), and (2) a chemical-mechanical based process (combination of peracetic acid and mechanical scraping). The developed decellularization processes were followed by additional hydrogen peroxide wash step. Optimal parameters were based on qualitative (histology and scanning electron microscopy) and quantitative (DNA and collagen assays, mechanical strength tests) studies. Results showed that the chemical-based decellularization process appears to be more effective in removing cellular elements and preserving ECM of porcine oesophagi than the chemical-mechanical based process. Furthermore, mechanical properties were mostly retained in the decellularized ECM scaffolds derived from chemical-based process. Master of Science (Biomedical Engineering) 2011-12-27T06:18:34Z 2011-12-27T06:18:34Z 2009 2009 Thesis http://hdl.handle.net/10356/47078 Nanyang Technological University application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
topic	DRNTU::Engineering::Bioengineering
spellingShingle	DRNTU::Engineering::Bioengineering Myint Zu Myaing. Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering
description	123 p.
author2	Chian Kerm Sin
author_facet	Chian Kerm Sin Myint Zu Myaing.
format	Theses and Dissertations
author	Myint Zu Myaing.
author_sort	Myint Zu Myaing.
title	Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering
title_short	Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering
title_full	Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering
title_fullStr	Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering
title_full_unstemmed	Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering
title_sort	biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering
publishDate	2011
url	http://hdl.handle.net/10356/47078
_version_	1761781455938125824

Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering

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