Isolation and culture of porcine smooth muscle cells on acellular porcine esophagus

89 p.

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Main Author: Wang, Lanying.
Other Authors: Chian Kerm Sin
Format: Theses and Dissertations
Published: 2011
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Online Access:http://hdl.handle.net/10356/47163
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-471632023-03-11T17:19:18Z Isolation and culture of porcine smooth muscle cells on acellular porcine esophagus Wang, Lanying. Chian Kerm Sin School of Mechanical and Aerospace Engineering DRNTU::Engineering::Bioengineering 89 p. Esophagus cancer has been causing high death rates in Asia. Current surgical procedures are highly stressful and often the cause of postoperative complications. Attempts to create an artificial esophagus have been made to address the need for esophagus reconstruction but none is clinically useful. Tissue engineering approach might provide potential solution for this matter. One of the promising methods in tissue engineering is to employ decellularized tissues (e.g. small intestine submucosa) as scaffolds to produce substitutes such as urinary tracts and blood vessels since these cell-free structures produce a microenvironment conducive to cell attachment, growth and reorganization. Therefore, it is believed that decellularized esophagus would be a potential solution for esophageal regeneration. The objectives of this study were (1) to isolate and harvest smooth muscle cells (SMCs) from porcine esophageal tissue and (2) to study and evaluate cell-matrix interactions of decellularized porcine esophageal matrices (DPEMs) seeded with porcine esophageal smooth muscle cells (PESMCs). Master of Science (Biomedical Engineering) 2011-12-27T06:30:39Z 2011-12-27T06:30:39Z 2005 2005 Thesis http://hdl.handle.net/10356/47163 Nanyang Technological University application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Wang, Lanying.
Isolation and culture of porcine smooth muscle cells on acellular porcine esophagus
description 89 p.
author2 Chian Kerm Sin
author_facet Chian Kerm Sin
Wang, Lanying.
format Theses and Dissertations
author Wang, Lanying.
author_sort Wang, Lanying.
title Isolation and culture of porcine smooth muscle cells on acellular porcine esophagus
title_short Isolation and culture of porcine smooth muscle cells on acellular porcine esophagus
title_full Isolation and culture of porcine smooth muscle cells on acellular porcine esophagus
title_fullStr Isolation and culture of porcine smooth muscle cells on acellular porcine esophagus
title_full_unstemmed Isolation and culture of porcine smooth muscle cells on acellular porcine esophagus
title_sort isolation and culture of porcine smooth muscle cells on acellular porcine esophagus
publishDate 2011
url http://hdl.handle.net/10356/47163
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