Prototype oligo array for direct transgene detection
70 p.
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2011
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| Online Access: | http://hdl.handle.net/10356/47443 |
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| Institution: | Nanyang Technological University |
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sg-ntu-dr.10356-474432023-02-28T18:45:26Z Prototype oligo array for direct transgene detection Muhammad Hidayat Sman Peter Rainer Preiser School of Biological Sciences DRNTU::Science::Biological sciences 70 p. We have developed an oligonucleotide microarray for detecting transgenic sequences in unamplified DNA. It consists of 390 probes, 27-33 bases long, targeting 100 sequences found in 6 commercial biotech crops. The probes were specific to conserved promoter/terminator sequences, genes for specific traits, selection markers and eventspecific sequences. A double-filtering strategy was used to design the probes and hybridizations were performed using an optimized catanionic solvent. Using this system, we were able to identify an unauthorized TT51-1 rice line (GenBank EU880444) containing previously undisclosed transgenic elements. Transgenic sequences copy numbers were determined by southern-blot analysis and sequence expression was confirmed by reverse-transcription PCR and sequencing. Analysis of 5% and 10% (w/w) transgenic rice and soybean samples showed that reliable detection depended on the copy numbers of target sequences and probe specificity. Our system detected multiple-copy inserts in 5% TT51-1 rice samples but not single-copy inserts in 10% GTS 40-3-2 soybean samples. Master of Science 2011-12-27T08:17:43Z 2011-12-27T08:17:43Z 2009 2009 Thesis http://hdl.handle.net/10356/47443 Nanyang Technological University application/pdf |
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DRNTU::Science::Biological sciences Muhammad Hidayat Sman Prototype oligo array for direct transgene detection |
| description |
70 p. |
| author2 |
Peter Rainer Preiser |
| author_facet |
Peter Rainer Preiser Muhammad Hidayat Sman |
| format |
Theses and Dissertations |
| author |
Muhammad Hidayat Sman |
| author_sort |
Muhammad Hidayat Sman |
| title |
Prototype oligo array for direct transgene detection |
| title_short |
Prototype oligo array for direct transgene detection |
| title_full |
Prototype oligo array for direct transgene detection |
| title_fullStr |
Prototype oligo array for direct transgene detection |
| title_full_unstemmed |
Prototype oligo array for direct transgene detection |
| title_sort |
prototype oligo array for direct transgene detection |
| publishDate |
2011 |
| url |
http://hdl.handle.net/10356/47443 |
| _version_ |
1759856742130253824 |