Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells

183 p.

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Bibliographic Details
Main Author:	Sui, Jianjun
Other Authors:	Ching Chi Bun
Format:	Theses and Dissertations
Published:	2011
Subjects:	DRNTU::Engineering::Chemical engineering
Online Access:	https://hdl.handle.net/10356/47463
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Institution:	Nanyang Technological University

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spelling	sg-ntu-dr.10356-474632023-03-03T15:58:53Z Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells Sui, Jianjun Ching Chi Bun Chen Wei Ning, William School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering 183 p. Propranolol is a nonselective beta-blocker exerting blocking effect on the beta-adrenergic receptors, with the S-enantiomer being more active than the R-enantiomer. Utilization of iTPvAQ coupled with two-dimensional LC-MS/MS system, we report here the first study of protein profiles in vascular smooth muscle cells (A7r5) in response to two individual enantiomers of propranolol, respectively. In this study four categories of cellular proteins including cytoskeletal proteins, signaling molecules, metabolic enzymes and those associated with DNA synthesis/protein translation showed differentially expressed protein levels. The higher protein levels of several enzymes involved in cellular anabolism and antioxidant activity in S-enantiomer of propranolol-incubated A7r5 cells, as revealed by LC MS/ MS, was further validated by real-time PCR. Significantly, the increase in the anabolic activity associated with the higher level of metabolic enzymes was also supported by the higher intracellular concentration of the metabolic cofactor NAD+, which was the product resulting from oxidation of NADH. The higher expression level of thioredoxin might account for increased antioxidant activity, as indicated by the lower ROS level in S-enantiomer of propranolol-incubated cells than that for R-enantiomer of propranolol-incubated cells and control ones. DOCTOR OF PHILOSOPHY (SCBE) 2011-12-27T08:22:43Z 2011-12-27T08:22:43Z 2009 2009 Thesis Sui, J. J. (2009). Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/47463 10.32657/10356/47463 Nanyang Technological University application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
topic	DRNTU::Engineering::Chemical engineering
spellingShingle	DRNTU::Engineering::Chemical engineering Sui, Jianjun Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells
description	183 p.
author2	Ching Chi Bun
author_facet	Ching Chi Bun Sui, Jianjun
format	Theses and Dissertations
author	Sui, Jianjun
author_sort	Sui, Jianjun
title	Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells
title_short	Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells
title_full	Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells
title_fullStr	Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells
title_full_unstemmed	Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells
title_sort	investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells
publishDate	2011
url	https://hdl.handle.net/10356/47463
_version_	1759853902257192960

Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells

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