Gene expression of cardiac differentiation of induced pluripotent stem cells.

As there is a lack of information on the cellular transcriptional regulation of cardiomyogenesis in hiPSCs, gene expression was investigated in viral-free hiPSC MSnviPSNF2 (C2) and MSnviPSNF3 (C3), with a novel cardiopoietic treatment employed for cardiac differentiation. Together with an overall in...

Full description

Saved in:
Bibliographic Details
Main Author: Ho, Wai Seen.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/10356/49489
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-49489
record_format dspace
spelling sg-ntu-dr.10356-494892023-02-28T18:02:14Z Gene expression of cardiac differentiation of induced pluripotent stem cells. Ho, Wai Seen. School of Biological Sciences National Heart Centre Singapore Ashish Mehta Winston Shim DRNTU::Science As there is a lack of information on the cellular transcriptional regulation of cardiomyogenesis in hiPSCs, gene expression was investigated in viral-free hiPSC MSnviPSNF2 (C2) and MSnviPSNF3 (C3), with a novel cardiopoietic treatment employed for cardiac differentiation. Together with an overall increase in spontaneous beat clusters formed in hiPSC-CMs, panel of 18 genetic markers has been evaluated using quantitative RNA expression analysis (qPCR). Pluripotency markers were down-regulated upon differentiation induction (d0-d2), followed by an up-regulation of mesodermal and cardiomesodermal genes (d2-d4), as well as cardiac-associated transcriptional factors (d2-d21). Then, an up-regulation of other cardiac-specific structural and ion channel markers was observed (d8-d21). Due to intrinsic and extrinsic factors during cardiac differentiation, both cell lines displayed distinctive variations and differentiation propensity in genetic expressions of the markers, such as the differential down regulation of Oct 4 and higher expression levels of Mesp1, Tbx5, Tbx20 and Isl1 in C3 differentiating EBs. Characterisation of undifferentiated hiPSC also revealed that both cell lines shared similar genetic and pluripotency capabilities to human embryonic stem cells (hESCs), thus proving to be a possible alternative to circumvent ethical and immunological issues of hESCs. Overall, these results provided genetic information for further modifications and optimizations for cardiac differentiation protocols. With higher quality and yield of hiPSC-CM, it will be easier to comply with clinical requirements for downstream processes in future personalized cardiac cell therapy treatments. Bachelor of Science in Biological Sciences 2012-05-21T02:33:47Z 2012-05-21T02:33:47Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49489 en Nanyang Technological University 33 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Ho, Wai Seen.
Gene expression of cardiac differentiation of induced pluripotent stem cells.
description As there is a lack of information on the cellular transcriptional regulation of cardiomyogenesis in hiPSCs, gene expression was investigated in viral-free hiPSC MSnviPSNF2 (C2) and MSnviPSNF3 (C3), with a novel cardiopoietic treatment employed for cardiac differentiation. Together with an overall increase in spontaneous beat clusters formed in hiPSC-CMs, panel of 18 genetic markers has been evaluated using quantitative RNA expression analysis (qPCR). Pluripotency markers were down-regulated upon differentiation induction (d0-d2), followed by an up-regulation of mesodermal and cardiomesodermal genes (d2-d4), as well as cardiac-associated transcriptional factors (d2-d21). Then, an up-regulation of other cardiac-specific structural and ion channel markers was observed (d8-d21). Due to intrinsic and extrinsic factors during cardiac differentiation, both cell lines displayed distinctive variations and differentiation propensity in genetic expressions of the markers, such as the differential down regulation of Oct 4 and higher expression levels of Mesp1, Tbx5, Tbx20 and Isl1 in C3 differentiating EBs. Characterisation of undifferentiated hiPSC also revealed that both cell lines shared similar genetic and pluripotency capabilities to human embryonic stem cells (hESCs), thus proving to be a possible alternative to circumvent ethical and immunological issues of hESCs. Overall, these results provided genetic information for further modifications and optimizations for cardiac differentiation protocols. With higher quality and yield of hiPSC-CM, it will be easier to comply with clinical requirements for downstream processes in future personalized cardiac cell therapy treatments.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Ho, Wai Seen.
format Final Year Project
author Ho, Wai Seen.
author_sort Ho, Wai Seen.
title Gene expression of cardiac differentiation of induced pluripotent stem cells.
title_short Gene expression of cardiac differentiation of induced pluripotent stem cells.
title_full Gene expression of cardiac differentiation of induced pluripotent stem cells.
title_fullStr Gene expression of cardiac differentiation of induced pluripotent stem cells.
title_full_unstemmed Gene expression of cardiac differentiation of induced pluripotent stem cells.
title_sort gene expression of cardiac differentiation of induced pluripotent stem cells.
publishDate 2012
url http://hdl.handle.net/10356/49489
_version_ 1759856283470528512