Studies on the host-virus interactions of coronaviruses

Virus-induced apoptosis and host antiviral innate immunity are key issues in understanding virus-host interactions and viral pathogenesis. Coronavirus infection of mammalian cells induces apoptotic and host innate immune responses. Global gene expression profiles are determined in coronavirus-infect...

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Main Author: Zhong, Yanxin
Other Authors: James P. Tam
Format: Theses and Dissertations
Language:English
Published: 2012
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Online Access:https://hdl.handle.net/10356/49502
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-495022023-02-28T18:45:37Z Studies on the host-virus interactions of coronaviruses Zhong, Yanxin James P. Tam Liu Ding Xiang School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology Virus-induced apoptosis and host antiviral innate immunity are key issues in understanding virus-host interactions and viral pathogenesis. Coronavirus infection of mammalian cells induces apoptotic and host innate immune responses. Global gene expression profiles are determined in coronavirus-infected Vero cells by Affymetrix array, using avian coronavirus infectious bronchitis virus (IBV) as a model system, to reveal up-regulation of both pro-apoptotic B cell lymphoma-2 (BCL-2)-antagonist/killer 1 (Bak) and pro-survival myeloid cell leukemia-1 (Mcl-1). Apoptosis occurred earlier in IBV-infected cells silenced with short interfering RNA targeting Mcl-1 (siMcl-1), and was delayed in siBak cells. Up-regulation of RNA helicases from the RIG-I-like receptor (RLR) family, Melanoma differentiation-associated gene 5 (MDA5) and Retinoic-inducible gene I (RIG-I), was also observed in IBV-infected cells, leading to downstream Mitochondrial antiviral signalling (MAVS) adaptor protein activation for interferon induction in response to viral invasion. RIG-I, MDA5 and MAVS also play a part in modulating IBV-induced apoptosis. Bcl-2 family proteins, too, regulates MAVS cleavage during apoptosis, thus further establishing a link between MAVS and intrinsic apoptotic pathway activation in the mitochondria during host innate immunity induction. DOCTOR OF PHILOSOPHY (SBS) 2012-05-21T04:04:18Z 2012-05-21T04:04:18Z 2012 2012 Thesis Zhong, Y. (2012). Studies on the host-virus interactions of coronaviruses. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/49502 10.32657/10356/49502 en 217 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Virology
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Virology
Zhong, Yanxin
Studies on the host-virus interactions of coronaviruses
description Virus-induced apoptosis and host antiviral innate immunity are key issues in understanding virus-host interactions and viral pathogenesis. Coronavirus infection of mammalian cells induces apoptotic and host innate immune responses. Global gene expression profiles are determined in coronavirus-infected Vero cells by Affymetrix array, using avian coronavirus infectious bronchitis virus (IBV) as a model system, to reveal up-regulation of both pro-apoptotic B cell lymphoma-2 (BCL-2)-antagonist/killer 1 (Bak) and pro-survival myeloid cell leukemia-1 (Mcl-1). Apoptosis occurred earlier in IBV-infected cells silenced with short interfering RNA targeting Mcl-1 (siMcl-1), and was delayed in siBak cells. Up-regulation of RNA helicases from the RIG-I-like receptor (RLR) family, Melanoma differentiation-associated gene 5 (MDA5) and Retinoic-inducible gene I (RIG-I), was also observed in IBV-infected cells, leading to downstream Mitochondrial antiviral signalling (MAVS) adaptor protein activation for interferon induction in response to viral invasion. RIG-I, MDA5 and MAVS also play a part in modulating IBV-induced apoptosis. Bcl-2 family proteins, too, regulates MAVS cleavage during apoptosis, thus further establishing a link between MAVS and intrinsic apoptotic pathway activation in the mitochondria during host innate immunity induction.
author2 James P. Tam
author_facet James P. Tam
Zhong, Yanxin
format Theses and Dissertations
author Zhong, Yanxin
author_sort Zhong, Yanxin
title Studies on the host-virus interactions of coronaviruses
title_short Studies on the host-virus interactions of coronaviruses
title_full Studies on the host-virus interactions of coronaviruses
title_fullStr Studies on the host-virus interactions of coronaviruses
title_full_unstemmed Studies on the host-virus interactions of coronaviruses
title_sort studies on the host-virus interactions of coronaviruses
publishDate 2012
url https://hdl.handle.net/10356/49502
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