Development of an immobilization-capture method for optical detection of bacteria

• Main Author: Er, Chin Lu
• Other Authors: Sun Delai, Darren
• Format: Theses and Dissertations
• Language: English
• Published: 2012
• Subjects: DRNTU::Engineering::Environmental engineering
• Online Access: http://hdl.handle.net/10356/50506
• Institution: Nanyang Technological University

There is a need to develop an online bacterial detection system which enables sample preparation, concentration and bacterial detection to be done in realtime. A flow immunosensor approach based on detection of the fluorescence emission has high potential to be developed into an online bacterial detector. In this study, we developed a system of immobilizing fluorescently-labelled antibody onto a glass surface where the cells are captured. This study aimed to develop an antibody immobilization method to immobilize fluorescently-labelled antibody specific to the target bacteria (E.coli) onto the glass surface to capture and label the cells. X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM) and epifluorescence microscopy studies revealed the feasibility of using aminopropyltriethoxysilane-glutaraldehyde (APTES-GA) to immobilize antibody onto glass substrates.