Electrochemical growth of MNO2 into hierarchical spheres for electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury.

• Main Author: Lin, Xi.
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Theses and Dissertations
• Language: English
• Published: 2012
• Subjects: DRNTU::Engineering::Chemical engineering
• Online Access: http://hdl.handle.net/10356/50535
• Institution: Nanyang Technological University

Using novel three-dimensional hierarchical spheres structure of MnO2 (MnO2 HS) as materials, an electrochemical immunosensor with the chemical immobilization of rabbit polygonal lipocalin-2 (LA2) antibody on MnO2 HS-modified glassy carbon electrode (GCE) is developed for neutrophil gelatinase-associated lipocalin (NGAL) detection without any label needed. The material was directly grown on the electrode using template-free electrochemical deposition under moderate conditions. The linear response range of the immunosensor is considerably wide (1ng/mL - 10μg/mL). The immunosensor achieved a low detection limit of 1ng/mL and also the potentiality to be used in the detection of NGAL in patient serum.