Purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes

Pugilina cochlidium is a marine snail that can lay a string of egg capsules made of an elastic biomaterial that encapsulates and protects the embryos from the harsh marine environments. Previous studies on Busycotypus canaliculatus, a different species of marine snail has found that the egg capsule...

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Main Author: Loke, Jun Jie
Other Authors: School of Materials Science and Engineering
Format: Final Year Project
Language:English
Published: 2013
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Online Access:http://hdl.handle.net/10356/51463
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-514632023-03-04T15:39:13Z Purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes Loke, Jun Jie School of Materials Science and Engineering Ali Gilles Tchenguise Miserez DRNTU::Engineering::Materials::Biomaterials Pugilina cochlidium is a marine snail that can lay a string of egg capsules made of an elastic biomaterial that encapsulates and protects the embryos from the harsh marine environments. Previous studies on Busycotypus canaliculatus, a different species of marine snail has found that the egg capsule is resilient and has shock absorbing properties. It exhibited elastic recovery which is non-entropy driven. The objective of this project is to extract and purify egg capsule precursor proteins found in the nidamental gland of Pugilina cochlidium, and characterise the microstructure of the egg capsule wall. The nidamental gland from a sedated Pugilina cochlidium was obtained by dissection. Reversed Phase – High Performance Liquid Chromatography (RP-HPLC) was then employed to separate the precursor proteins. Individual fractions collected from the RP-HPLC were subjected to Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis (SDS-PAGE) to determine the purity. Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI-TOF MS) was used to further determine the molecular weights of the proteins. Optical microscope (OM), scanning electron microscope (SEM) and atomic force microscope (AFM) were used to characterise the structure of the egg capsule. Fibres were observed to have a banding periodicity. The mean values of the banding periodicity and fibre thickness were obtained. Bachelor of Engineering (Materials Engineering) 2013-04-03T04:14:43Z 2013-04-03T04:14:43Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/51463 en Nanyang Technological University 41 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Loke, Jun Jie
Purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes
description Pugilina cochlidium is a marine snail that can lay a string of egg capsules made of an elastic biomaterial that encapsulates and protects the embryos from the harsh marine environments. Previous studies on Busycotypus canaliculatus, a different species of marine snail has found that the egg capsule is resilient and has shock absorbing properties. It exhibited elastic recovery which is non-entropy driven. The objective of this project is to extract and purify egg capsule precursor proteins found in the nidamental gland of Pugilina cochlidium, and characterise the microstructure of the egg capsule wall. The nidamental gland from a sedated Pugilina cochlidium was obtained by dissection. Reversed Phase – High Performance Liquid Chromatography (RP-HPLC) was then employed to separate the precursor proteins. Individual fractions collected from the RP-HPLC were subjected to Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis (SDS-PAGE) to determine the purity. Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI-TOF MS) was used to further determine the molecular weights of the proteins. Optical microscope (OM), scanning electron microscope (SEM) and atomic force microscope (AFM) were used to characterise the structure of the egg capsule. Fibres were observed to have a banding periodicity. The mean values of the banding periodicity and fibre thickness were obtained.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Loke, Jun Jie
format Final Year Project
author Loke, Jun Jie
author_sort Loke, Jun Jie
title Purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes
title_short Purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes
title_full Purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes
title_fullStr Purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes
title_full_unstemmed Purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes
title_sort purification and self-assembly of natural coiled-coil proteins from marine snail pugilina cochlidium egg case membranes
publishDate 2013
url http://hdl.handle.net/10356/51463
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