Electrospun poly(caprolactone) (PCL) microfibers with controlled release of ascorbic acid for bone tissue engineering

The concept of bone tissue engineering holds great promise for the future treatment of large bone defects through the utility of implanting degradable polymeric scaffolds, to which mesenchymal stem cells (MSCs) can attach, allowing the ingrowths of new tissue. Current methods of osteoinduction of...

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Main Author: Toh, Jessica Pei Wen.
Other Authors: Loo Say Chye Joachim
Format: Final Year Project
Language:English
Published: 2013
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Online Access:http://hdl.handle.net/10356/51837
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-518372023-03-04T15:35:08Z Electrospun poly(caprolactone) (PCL) microfibers with controlled release of ascorbic acid for bone tissue engineering Toh, Jessica Pei Wen. Loo Say Chye Joachim School of Materials Science and Engineering DRNTU::Engineering The concept of bone tissue engineering holds great promise for the future treatment of large bone defects through the utility of implanting degradable polymeric scaffolds, to which mesenchymal stem cells (MSCs) can attach, allowing the ingrowths of new tissue. Current methods of osteoinduction of cultured stem cells require osteogenic supplements to be replenished every 2 to 3 days. Although it is convenient to do so for in vitro, it will cause unnecessary pain and inconvenience for in vivo testing. Thus, the purpose of this project is to realize a sustained release of ascorbic acid (one of the osteogenic supplements), which was loaded into the scaffold directly, up to 14 days to induce early osteogenic differentiation in vitro. Poly(caprolactone) (PCL) scaffold of micro-sized fibers with embedded ascorbic acid was being fabricated through electrospinning method. By manipulating processing factors, such as electrospinning conditions and polymer solution parameters, drug delivery profiles of ascorbic acid, fiber morphology and mechanical properties of scaffolds can be optimized. Drug release profiles were studied by UV-Vis spectroscopy, while fiber morphology and mechanical properties of scaffolds were studied using FESEM and instron tester respectively. Different solvents systems (DCM + DMF or DCM + Methanol), spinning methods (core-sheath, single and emulsion) and the addition of polymer or copolymers were tested to find the best ascorbic acid (ASB) release profile for scaffolds. It was observed that the emulsion spinning method gave the best result of obtaining micro-sized fibers with drug release profile of 40% initial burst release and up to 70% of sustained release of ASB over 14 days. However, its young‟s modulus was found to be the lowest as compared to other experimented scaffolds. Thus, more work needs to be done to find a more appropriate scaffold for bone tissue engineering. Bachelor of Engineering (Materials Engineering) 2013-04-11T07:06:10Z 2013-04-11T07:06:10Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/51837 en Nanyang Technological University 49 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Toh, Jessica Pei Wen.
Electrospun poly(caprolactone) (PCL) microfibers with controlled release of ascorbic acid for bone tissue engineering
description The concept of bone tissue engineering holds great promise for the future treatment of large bone defects through the utility of implanting degradable polymeric scaffolds, to which mesenchymal stem cells (MSCs) can attach, allowing the ingrowths of new tissue. Current methods of osteoinduction of cultured stem cells require osteogenic supplements to be replenished every 2 to 3 days. Although it is convenient to do so for in vitro, it will cause unnecessary pain and inconvenience for in vivo testing. Thus, the purpose of this project is to realize a sustained release of ascorbic acid (one of the osteogenic supplements), which was loaded into the scaffold directly, up to 14 days to induce early osteogenic differentiation in vitro. Poly(caprolactone) (PCL) scaffold of micro-sized fibers with embedded ascorbic acid was being fabricated through electrospinning method. By manipulating processing factors, such as electrospinning conditions and polymer solution parameters, drug delivery profiles of ascorbic acid, fiber morphology and mechanical properties of scaffolds can be optimized. Drug release profiles were studied by UV-Vis spectroscopy, while fiber morphology and mechanical properties of scaffolds were studied using FESEM and instron tester respectively. Different solvents systems (DCM + DMF or DCM + Methanol), spinning methods (core-sheath, single and emulsion) and the addition of polymer or copolymers were tested to find the best ascorbic acid (ASB) release profile for scaffolds. It was observed that the emulsion spinning method gave the best result of obtaining micro-sized fibers with drug release profile of 40% initial burst release and up to 70% of sustained release of ASB over 14 days. However, its young‟s modulus was found to be the lowest as compared to other experimented scaffolds. Thus, more work needs to be done to find a more appropriate scaffold for bone tissue engineering.
author2 Loo Say Chye Joachim
author_facet Loo Say Chye Joachim
Toh, Jessica Pei Wen.
format Final Year Project
author Toh, Jessica Pei Wen.
author_sort Toh, Jessica Pei Wen.
title Electrospun poly(caprolactone) (PCL) microfibers with controlled release of ascorbic acid for bone tissue engineering
title_short Electrospun poly(caprolactone) (PCL) microfibers with controlled release of ascorbic acid for bone tissue engineering
title_full Electrospun poly(caprolactone) (PCL) microfibers with controlled release of ascorbic acid for bone tissue engineering
title_fullStr Electrospun poly(caprolactone) (PCL) microfibers with controlled release of ascorbic acid for bone tissue engineering
title_full_unstemmed Electrospun poly(caprolactone) (PCL) microfibers with controlled release of ascorbic acid for bone tissue engineering
title_sort electrospun poly(caprolactone) (pcl) microfibers with controlled release of ascorbic acid for bone tissue engineering
publishDate 2013
url http://hdl.handle.net/10356/51837
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