Biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail Busycotypus canaliculatus egg case membranes

Due to the harsh environment of its natural habitat, the eggs of Busycotypus canaliculatus have developed a unique shock-absorbing property, protecting the embryos from harm. This material is of interest in the biomimetic materials engineering field as a bio-encapsulant as its properties make it sui...

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Main Author: Tan, Delford Kang Hong
Other Authors: School of Materials Science and Engineering
Format: Final Year Project
Language:English
Published: 2013
Subjects:
Online Access:http://hdl.handle.net/10356/51852
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-518522023-03-04T15:34:09Z Biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail Busycotypus canaliculatus egg case membranes Tan, Delford Kang Hong School of Materials Science and Engineering Ali Gilles Tchenguise Miserez DRNTU::Engineering::Materials::Biomaterials Due to the harsh environment of its natural habitat, the eggs of Busycotypus canaliculatus have developed a unique shock-absorbing property, protecting the embryos from harm. This material is of interest in the biomimetic materials engineering field as a bio-encapsulant as its properties make it suitable for the protection of delicate cells and tissues during transplantation. This project aims to study the egg capsules of the Busycotypus canaliculatus in order to understand the self-assembly process of the egg capsule proteins. Purification of the capsule proteins was carried out using the high performance liquid chromatography, while characterisation of the proteins was done with mass spectroscopy. The structure of the egg capsules was investigated with scanning electron microscopy and fourier transform infrared microscopy. Purification of the capsule proteins was largely successful, although further work is needed to obtain purified samples of all four capsule proteins. The images from scanning electron microscopy revealed banding patterns reminiscent of collagen fibre bandings, while fourier transform infrared microscopy shed light on the composition of the secondary structures in the capsule proteins. Bachelor of Engineering (Materials Engineering) 2013-04-11T07:49:18Z 2013-04-11T07:49:18Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/51852 en Nanyang Technological University 34 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Tan, Delford Kang Hong
Biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail Busycotypus canaliculatus egg case membranes
description Due to the harsh environment of its natural habitat, the eggs of Busycotypus canaliculatus have developed a unique shock-absorbing property, protecting the embryos from harm. This material is of interest in the biomimetic materials engineering field as a bio-encapsulant as its properties make it suitable for the protection of delicate cells and tissues during transplantation. This project aims to study the egg capsules of the Busycotypus canaliculatus in order to understand the self-assembly process of the egg capsule proteins. Purification of the capsule proteins was carried out using the high performance liquid chromatography, while characterisation of the proteins was done with mass spectroscopy. The structure of the egg capsules was investigated with scanning electron microscopy and fourier transform infrared microscopy. Purification of the capsule proteins was largely successful, although further work is needed to obtain purified samples of all four capsule proteins. The images from scanning electron microscopy revealed banding patterns reminiscent of collagen fibre bandings, while fourier transform infrared microscopy shed light on the composition of the secondary structures in the capsule proteins.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Tan, Delford Kang Hong
format Final Year Project
author Tan, Delford Kang Hong
author_sort Tan, Delford Kang Hong
title Biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail Busycotypus canaliculatus egg case membranes
title_short Biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail Busycotypus canaliculatus egg case membranes
title_full Biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail Busycotypus canaliculatus egg case membranes
title_fullStr Biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail Busycotypus canaliculatus egg case membranes
title_full_unstemmed Biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail Busycotypus canaliculatus egg case membranes
title_sort biomimetic encapsulant : purification and self-assembly of natural coiled-coil proteins from marine snail busycotypus canaliculatus egg case membranes
publishDate 2013
url http://hdl.handle.net/10356/51852
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