Overexpression of RasV12 and its role in promoting the robustness of HEK293T cells.

• Main Author: Chow, Peiyi Vicki.
• Other Authors: School of Biological Sciences
• Format: Final Year Project
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences::Molecular biology
• Online Access: http://hdl.handle.net/10356/52745
• Institution: Nanyang Technological University

Mammalian cells are usually grown in stirred bioreactors for the production of biopharmaceuticals in which the proliferation and health of the cells are affected by the limiting factors such as environment and growth inhibition. To overcome such limitations, cell engineering, such as the overexpress...