Synergetic effect of cysteine rich protein and chemotherapeutic drugs.

• Main Author: Wong, Liling.
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Final Year Project
• Language: English
• Published: 2013
• Subjects: DRNTU::Engineering::Bioengineering
• Online Access: http://hdl.handle.net/10356/52860
• Institution: Nanyang Technological University

There are several commercial drugs marketed as chemotherapy drugs to treat cancer such as Taxol, Etoposide and Vincristine. These drugs arrest cancer cells growth by inhibiting mitosis, thus resulting in apoptosis rather than proliferation. Recent discoveries of twelve new proteins derived from Clitoria Ternatea are rich in cysteine, hence they are also known as Cystein Rich Protein (CRP). Reports have shown that they have anti-cancer effects and these CRP will be one of our focus areas. This report also focuses on a type CRP, SFBK, which is chemically engineered from a portion of SFTI-1 and bradykinin. To test its bioactivity, MTT assay was performed on them through a combination of commercial chemotherapeutic drugs on a Hela-C3 carcinoma cells. MTT values obtained will be used to determine any synergetic effect. It is found that SFBK and CT20 yield low synergetic effect with chemotherapeutic drugs but CT9 has high synergetic effect with chemotherapeutic drugs.