Kinetic analysis of IL-4 and IL-13 production in chronic experimental asthma.
IL-4 and IL-13 cytokines secreted by T helper 2 cells are deeply implicated in the pathogenesis of asthma. The comprehension of the kinetics of IL-4 and IL-13 cytokines and its signaling pathway may assist in the formulation of treatment strategies targeting these Th2 cytokines. In this study, we ai...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2013
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| Online Access: | http://hdl.handle.net/10356/53791 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | IL-4 and IL-13 cytokines secreted by T helper 2 cells are deeply implicated in the pathogenesis of asthma. The comprehension of the kinetics of IL-4 and IL-13 cytokines and its signaling pathway may assist in the formulation of treatment strategies targeting these Th2 cytokines. In this study, we aim to determine the kinetics of IL-4 and IL-13 production, as well as their downstream targets, during the progression of experimental asthma. We utilize a model of experimental asthma induced in A/J mice via intranasal administration of chicken ovalbumin (OVA). We found that the production of IL-4 protein progressively increases in the airways during the four weeks OVA treatment, while IL-13 production reaches a plateau at two weeks of OVA treatment and is maintained thereafter. Using real time PCR analysis, we also detected an increase in downstream targets of IL-4/IL-13, such as IL-13Rα2, CCL11 and Muc5ac after OVA treatment. |
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