Systems biology analysis of plasmodium host parasite interactions.

• Main Author: Wong, Lu Yi.
• Other Authors: School of Biological Sciences
• Format: Final Year Project
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences::Molecular biology
• Online Access: http://hdl.handle.net/10356/53803
• Institution: Nanyang Technological University

Plasmodium Falciparum, is the main cause of the disease malaria which kills millions especially in the sub-Saharan region annually. This project (preliminary study) aims to annotate the many plasmodium gene with unknown functions using the guilt by association method. To achieve this aim, we perturb the parasite at trophozoite stage only (3D7 strain) with 400 compounds from the Malaria Box collection and perform microarray. The resulting 41 (out of 400) expression profiles profiles are used to generate a co expression network for further analysis. A previous study have shown chemical perturbation to produce relevant and reproducible gene profiles. If successful, this will validate the accuracy and feasibility of using drug perturbation together with microarrays to elucidate gene functions in a fast and comprehensible manner. The resultant network clusters show some biological relevance, especially the “invasion” cluster.