Feasibility study of a biochip for mumps virus enzyme-linked immunosorbent assay (ELISA)

• Main Author: Woon, Fung Peng.
• Other Authors: Gong, Thomas Haiqing
• Format: Theses and Dissertations
• Published: 2008
• Subjects: DRNTU::Engineering::Mechanical engineering::Bio-mechatronics
• Online Access: http://hdl.handle.net/10356/5399
• Institution: Nanyang Technological University

Plastic chips were fabricated for the purpose of creating a lab-on-a-chip to miniaturise the Mumps virus enzyme-linked immunosorbent assay (ELISA). The pattern of microfluidic channels was first transferred from a glass master template possessing an inverse image of the channels to polystyrene chips which were then bonded to polycarbonate covers. A good imprint of the channels and wells could be obtained at a hot embossing temperature of 110°C and by holding the press for 3 minutes. The chip could be tightly bonded to its cover at 110 °C while maintaining the press for 3 minutes. Results showed that it was possible to reduce the volume of reagents used and the time taken to perform the immunoassay on the biochip as compared with performing it on microtiter wells supplied in commercial test kits.