Shape memory effect of NOA-63 polymer and its application in cell culture

Cell culture substrates of defined topography have emerged as powerful tools to investigate cell growth and proliferation. However, current technologies only allow passive control of substrate properties. Hence a thermo-responsive cell culture system that uses shape memory polymer (SMP) substrates w...

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Main Author: Chua, Yuan Hong.
Other Authors: School of Mechanical and Aerospace Engineering
Format: Final Year Project
Language:English
Published: 2013
Subjects:
Online Access:http://hdl.handle.net/10356/54013
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-540132023-03-04T18:46:04Z Shape memory effect of NOA-63 polymer and its application in cell culture Chua, Yuan Hong. School of Mechanical and Aerospace Engineering Zhang YiLei DRNTU::Engineering::Mechanical engineering Cell culture substrates of defined topography have emerged as powerful tools to investigate cell growth and proliferation. However, current technologies only allow passive control of substrate properties. Hence a thermo-responsive cell culture system that uses shape memory polymer (SMP) substrates was introduced as the substrates can be programmed to change surface topography during cell culture. It was presumed that a shape memory-activated change in substrate topography could be used to control cell behaviour. To test this hypothesis, several tests and experiments were carried out to prove that shape memory properties exist in the chosen polymer. A flat SMP substrate was then embossed with patterns to produce a temporary topography of parallel micron-scale grooves. After plating cells on the substrate, shape recovery was triggered using a change in temperature tailored to be compatible with plant cell culture, thereby causing topographic recovery of the substrate back to its original flat surface. Alignment of the cell body and microfilaments along the grooves of the temporary topography was significantly improved using the substrate. These results demonstrate control of cell behaviour using shape-memory-activated topographic changes. This facilitates the investigation of cell biomechanical function, and cell soft-matter physics with the use of active cell culture. Bachelor of Engineering (Mechanical Engineering) 2013-06-11T07:06:16Z 2013-06-11T07:06:16Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/54013 en Nanyang Technological University 66 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Mechanical engineering
spellingShingle DRNTU::Engineering::Mechanical engineering
Chua, Yuan Hong.
Shape memory effect of NOA-63 polymer and its application in cell culture
description Cell culture substrates of defined topography have emerged as powerful tools to investigate cell growth and proliferation. However, current technologies only allow passive control of substrate properties. Hence a thermo-responsive cell culture system that uses shape memory polymer (SMP) substrates was introduced as the substrates can be programmed to change surface topography during cell culture. It was presumed that a shape memory-activated change in substrate topography could be used to control cell behaviour. To test this hypothesis, several tests and experiments were carried out to prove that shape memory properties exist in the chosen polymer. A flat SMP substrate was then embossed with patterns to produce a temporary topography of parallel micron-scale grooves. After plating cells on the substrate, shape recovery was triggered using a change in temperature tailored to be compatible with plant cell culture, thereby causing topographic recovery of the substrate back to its original flat surface. Alignment of the cell body and microfilaments along the grooves of the temporary topography was significantly improved using the substrate. These results demonstrate control of cell behaviour using shape-memory-activated topographic changes. This facilitates the investigation of cell biomechanical function, and cell soft-matter physics with the use of active cell culture.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Chua, Yuan Hong.
format Final Year Project
author Chua, Yuan Hong.
author_sort Chua, Yuan Hong.
title Shape memory effect of NOA-63 polymer and its application in cell culture
title_short Shape memory effect of NOA-63 polymer and its application in cell culture
title_full Shape memory effect of NOA-63 polymer and its application in cell culture
title_fullStr Shape memory effect of NOA-63 polymer and its application in cell culture
title_full_unstemmed Shape memory effect of NOA-63 polymer and its application in cell culture
title_sort shape memory effect of noa-63 polymer and its application in cell culture
publishDate 2013
url http://hdl.handle.net/10356/54013
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