Design, construction and characterization of a microfluidic chip
Microfluidic chips have paved the way for efficient and economical diagnostics of DNA in the biological field. This report reviews a novel Microfluidic Liquid Phase Nucleic Acid Purification Chip that is able to extract DNA/RNA from a sample. This is done by flushing a reagent in a forward...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/54118 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Microfluidic chips have paved the way for efficient and economical diagnostics of
DNA in the biological field. This report reviews a novel Microfluidic Liquid Phase
Nucleic Acid Purification Chip that is able to extract DNA/RNA from a sample. This
is done by flushing a reagent in a forward and back manner over the wells containing
the sample, which will leave behind the purified DNA/RNA in the wells after the
process. However, there were a few possibilities not addressed, like post-extraction
protocol, the collection of the purified lysate from the wells and the study of flow
rate in multiple channels. This report will investigate the fabrication, loading
procedures and the possible design improvements for post-extraction protocol and
investigation of the consistency of flow rates. The report will also investigate the
potential of size based cell/particle enrichment by varying factors like flow rate and
cycles of flushing motion.
Lastly, the report will present an improved design and possible fabrication process
using the results of the investigation. |
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