Study mechanism of metastasis of triple-negitive breast cancer cells through proteomics.

• Main Author: Luo, Man.
• Other Authors: Luo Qian Kathy
• Format: Theses and Dissertations
• Language: English
• Published: 2013
• Subjects: DRNTU::Engineering::Bioengineering
• Online Access: http://hdl.handle.net/10356/54308
• Institution: Nanyang Technological University

Three triple-negative breast cancer cell lines have been generated from MDA-MB-231 cells in Prof. Kathy Qian Luo’s Lab, including 231-C3, 231-M1 and 231-M2 cells. MCF-7-C3 cell line has also been generated from MCF-7 cells which express estrogen receptor (ER), progesterone receptor (PR) compared with MDA-MB-231 cells. These cell lines were found with different survival ability in zebrafish model and with UV light, Doxorubicin and H2O2 treatment. Given that UV light and Doxorubicin are both reactive oxygen species (ROS) generator and the pulsatile flow of zebrafish blood circulation can also induce ROS production, a hypothesis was formed that resistance to oxidative stress should be a possible mechanism for the strong metastatic potential for triple-negative breast cancer cells. In this project, 231-M1 and 231-M2 cells can survive the oxidant stress better than the parental 231-C3 cells, so these two cell lines should have stronger antioxidant ability.