Study of cell apoptosis during cancer metastasis in zebrafish model.

Resistance to apoptosis is one of the super powers for malignant cancer cells to survive through the complicated metastatic process. In our recent study, we found that high malignant cancer cells have resistance to apoptotic stimuli from the blood circulation. From these results, we hypothesize that...

Full description

Saved in:
Bibliographic Details
Main Author: Ngan Weida.
Other Authors: Kathy Luo Qian
Format: Final Year Project
Language:English
Published: 2013
Subjects:
Online Access:http://hdl.handle.net/10356/54563
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-54563
record_format dspace
spelling sg-ntu-dr.10356-545632023-03-03T15:41:26Z Study of cell apoptosis during cancer metastasis in zebrafish model. Ngan Weida. Kathy Luo Qian School of Chemical and Biomedical Engineering DRNTU::Science::Biological sciences::Zoology::Vertebrates Resistance to apoptosis is one of the super powers for malignant cancer cells to survive through the complicated metastatic process. In our recent study, we found that high malignant cancer cells have resistance to apoptotic stimuli from the blood circulation. From these results, we hypothesize that blood circulation is a very important obstacle for cancer metastasis and thus it’s should be a best window for testing of anti-metastasis treatment. In this project, caspase-3 FRET sensor is used for detecting tumor cell apoptosis in the circulation of zebrafish embryo. The B16F10-C3 cells displayed strong resistance to apoptosis even in the presence of PI3K inhibitor, LY294002. The pre-treatment of LY294002 appeared to have down regulated adhesion integrins of the tumor cells resulting in the cell to cell adhesion to be weakened resulting in a higher percentage of cells to be arrested in the intersegmental vessel. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2013-06-24T03:06:45Z 2013-06-24T03:06:45Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/54563 en Nanyang Technological University 55 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Zoology::Vertebrates
spellingShingle DRNTU::Science::Biological sciences::Zoology::Vertebrates
Ngan Weida.
Study of cell apoptosis during cancer metastasis in zebrafish model.
description Resistance to apoptosis is one of the super powers for malignant cancer cells to survive through the complicated metastatic process. In our recent study, we found that high malignant cancer cells have resistance to apoptotic stimuli from the blood circulation. From these results, we hypothesize that blood circulation is a very important obstacle for cancer metastasis and thus it’s should be a best window for testing of anti-metastasis treatment. In this project, caspase-3 FRET sensor is used for detecting tumor cell apoptosis in the circulation of zebrafish embryo. The B16F10-C3 cells displayed strong resistance to apoptosis even in the presence of PI3K inhibitor, LY294002. The pre-treatment of LY294002 appeared to have down regulated adhesion integrins of the tumor cells resulting in the cell to cell adhesion to be weakened resulting in a higher percentage of cells to be arrested in the intersegmental vessel.
author2 Kathy Luo Qian
author_facet Kathy Luo Qian
Ngan Weida.
format Final Year Project
author Ngan Weida.
author_sort Ngan Weida.
title Study of cell apoptosis during cancer metastasis in zebrafish model.
title_short Study of cell apoptosis during cancer metastasis in zebrafish model.
title_full Study of cell apoptosis during cancer metastasis in zebrafish model.
title_fullStr Study of cell apoptosis during cancer metastasis in zebrafish model.
title_full_unstemmed Study of cell apoptosis during cancer metastasis in zebrafish model.
title_sort study of cell apoptosis during cancer metastasis in zebrafish model.
publishDate 2013
url http://hdl.handle.net/10356/54563
_version_ 1759858374984335360